지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.2
- 수록면
- 61 - 68 (8page)
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Entamoeba histolytica, which causes amoebic colitis and occasionally liver abscess in humans, is able to induce host cell death. However, signaling mechanisms of colon cell death induced by E. histolytica are not fully elucidated. In this study, we investigated the signaling role of NOX in cell death of HT29 colonic epithelial cells induced by E. histolytica. Incubation of HT29 cells with amoebic trophozoites resulted in DNA fragmentation that is a hallmark of apoptotic cell death. In addition, E. histolytica generate intracellular reactive oxygen species (ROS) in a contact-dependent manner. Inhibition of intracellular ROS level with treatment with DPI, an inhibitor of NADPH oxidases (NOXs), decreased Entamoeba-induced ROS generation and cell death in HT29 cells. However, pan-caspase inhibitor did not affect E. histolytica-induced HT29 cell death. In HT29 cells, catalytic subunit NOX1 and regulatory subunit Rac1 for NOX1 activation were highly expressed. We next investigated whether NADPH oxidase 1 (NOX1)-derived ROS is closely associated with HT29 cell death induced by E. histolytica. Suppression of Rac1 by siRNA significantly inhibited Entamoeba-induced cell death. Moreover, knockdown of NOX1 by siRNA, effectively inhibited E. histolytica-triggered DNA fragmentation in HT29 cells. These results suggest that NOX1-derived ROS is required for apoptotic cell death in HT29 colon epithelial cells induced by E. histolytica.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (32)
참고문헌 신청
1. [학술지(정기간행물)] - Stanley SL Jr / 2003 / Amoebiasis / Lancet 361 : 1025 ~ 1034
2. [학술지(정기간행물)] - Moncada D / 2006 / Antisense inhibition of Entamoeba histolytica cysteine proteases inhibits colonic mucus degradation / Gastroenterology 130 : 721 ~ 730
3. [학술지(정기간행물)] - Petri WA / 2002 / The bittersweet interface of parasite and host: lectin-carbohydrate interactions during human invasion by the parasite Entamoeba histolytica / Annu Rev Microbiol 56 : 39 ~ 64
4. [학술지(정기간행물)] - Berninghausen O / 1997 / Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica / Infect Immun 65 : 3615 ~ 3621
5. [학술지(정기간행물)] - Sim S / 2007 / Involvement of beta 2-integrin in ROS-mediated neutrophil apoptosis induced by Entamoeba histolytica / Microbes Infect 9 : 1368 ~ 1375
2. [학술지(정기간행물)] - Moncada D / 2006 / Antisense inhibition of Entamoeba histolytica cysteine proteases inhibits colonic mucus degradation / Gastroenterology 130 : 721 ~ 730
3. [학술지(정기간행물)] - Petri WA / 2002 / The bittersweet interface of parasite and host: lectin-carbohydrate interactions during human invasion by the parasite Entamoeba histolytica / Annu Rev Microbiol 56 : 39 ~ 64
4. [학술지(정기간행물)] - Berninghausen O / 1997 / Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica / Infect Immun 65 : 3615 ~ 3621
5. [학술지(정기간행물)] - Sim S / 2007 / Involvement of beta 2-integrin in ROS-mediated neutrophil apoptosis induced by Entamoeba histolytica / Microbes Infect 9 : 1368 ~ 1375
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UCI(KEPA) : I410-ECN-0101-2014-510-002535695