지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.4
- 수록면
- 227 - 247 (21page)
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초록· 키워드
오류제보하기
MicroRNAs (miRNAs) are a class of recently discovered non-coding small RNA molecules, on average approximately 21 nucleotides in length, which underlie numerous important biological roles in gene regulation in various organisms. The miRNA database (release 18) has 18,226 miRNAs, which have been deposited from different species. Although miRNAs have been identified and validated in many plant species, no studies have been reported on discovering miRNAs in Panax ginseng Meyer, which is a traditionally known medicinal plant in oriental medicine, also known as Korean ginseng. It has triterpene ginseng saponins called ginsenosides, which are responsible for its various pharmacological activities. Predicting conserved miRNAs by homologybased analysis with available expressed sequence tag (EST) sequences can be powerful, if the species lacks whole genome sequence information. In this study by using the EST based computational approach, 69 conserved miRNAs belonging to 44 miRNA families were identified in Korean ginseng. The digital gene expression patterns of predicted conserved miRNAs were analyzed by deep sequencing using small RNA sequences of flower buds, leaves, and lateral roots. We have found that many of the identified miRNAs showed tissue specific expressions. Using the insilico method, 346 potential targets were identified for the predicted 69 conserved miRNAs by searching the ginseng EST database, and the predicted targets were mainly involved in secondary metabolic processes, responses to biotic and abiotic stress, and transcription regulator activities, as well as a variety of other metabolic processes.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
Appendix
참고문헌 (50)
참고문헌 신청
1. [학술지(정기간행물)] - Bartel DP. / 2004 / MicroRNAs: genomics, biogenesis, mechanism, and function / Cell 116 : 281 ~ 297
2. [학술지(정기간행물)] - Zhou X / 2007 / Characterization and identification of microRNA core promoters in four model species / PLoS Comput Biol 3 : e37 ~
3. [학술지(정기간행물)] - Jones-Rhoades MW / 2006 / MicroRNAS and their regulatory roles in plants / Annu Rev Plant Biol 57 : 19 ~ 53
4. [학술지(정기간행물)] - Lu Y / 2010 / Computational identification of novel microRNAs and their targets in Vigna unguiculata / Comp Funct Genomics pii : 128297 ~
5. [학술지(정기간행물)] - Sunkar R / 2004 / Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis / Plant Cell 16 : 2001 ~ 2019
2. [학술지(정기간행물)] - Zhou X / 2007 / Characterization and identification of microRNA core promoters in four model species / PLoS Comput Biol 3 : e37 ~
3. [학술지(정기간행물)] - Jones-Rhoades MW / 2006 / MicroRNAS and their regulatory roles in plants / Annu Rev Plant Biol 57 : 19 ~ 53
4. [학술지(정기간행물)] - Lu Y / 2010 / Computational identification of novel microRNAs and their targets in Vigna unguiculata / Comp Funct Genomics pii : 128297 ~
5. [학술지(정기간행물)] - Sunkar R / 2004 / Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis / Plant Cell 16 : 2001 ~ 2019
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UCI(KEPA) : I410-ECN-0101-2014-520-003018788