Objectives: The purpose of this study was to analyze the relationship between Yin-deficiency questionnaire score and various biofunctional signals in women. Methods: A retrospective chart review was performed on charts of 195 patients who visited Gangdong Kyung Hee Hospital between April 1st and September 30th, 2011. The subjects were categorized into two groups, a low Yin-deficiency group (n=118) and a high Yin-deficiency group (n=77). The authors analyzed the correlation between Yin-deficiency questionnaire score and biofunctional signals by Pearson"s correlation coefficient test and the difference in biofunctional signals between the two groups by independent samples t-test using SPSS for windows. Results: 1. Negative correlations were observed between the temperature difference of back-humerus, standard deviation of all R-R intervals (SDNN), total power (TP), low frequency (LF), high frequency (HF) on heart rate variability parameters, and Yin-deficiency questionnaire score. A positive correlation was observed between the temperature difference of knee-humerus and Yin-deficiency questionnaire score. 2. The temperature difference of back-humerus in the high Yin-deficiency group was significantly higher than that in the low Yin-deficiency group. The temperature difference of knee-humerus, height, waist-hip ratio, SDNN, TP, LF, and HF of the high Yin-deficiency group were significantly lower than those of the low Yin-deficiency group. Conclusions: The results of this study suggest that the comprehensive diagnosis of Yin-deficiency and biofunctional signals is useful.