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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objective: This study was designed to investigate the effect of Samultang (SMT) under hippocampus cells ischemia both in vitro and in vivo.
Methods: In the in vitro study, HT22 cells, predominantly detected in the cytoplasm, which coincides with the location of the mitochondria, were used as indicators. In the in vivo study, permanent middle cerebral artery occlusion (MCAO) was induced on rats. SMT was given orally 2 h before induction of permanent focal brain ischemic injury.
Result: In the in vitro study, SMT had protective effects in glutamate-induced cytotoxicity, which was revealed as apoptosis characterized by chromatic condensation and the loss of mitochondrial membrane potential in HT22 cells. In the in vivo study, TTC (2,3,5-triphenyltetrazolium chloride) staining showed a marked ischemic injury in blood supply territory of the middle cerebral artery (MCA) such as the cerebral cortex and striatum. However, treatment with SMT significantly reduced infarcted volume. SMT increased marked survival of HT22 cells against glutamate-induced cytotoxicity in MTT assay.
Conclusion: These results suggest that water extract of SMT provides neuroprotection against ischemic or oxidative injury by inhibition of apoptotic cell death.
Methods: In the in vitro study, HT22 cells, predominantly detected in the cytoplasm, which coincides with the location of the mitochondria, were used as indicators. In the in vivo study, permanent middle cerebral artery occlusion (MCAO) was induced on rats. SMT was given orally 2 h before induction of permanent focal brain ischemic injury.
Result: In the in vitro study, SMT had protective effects in glutamate-induced cytotoxicity, which was revealed as apoptosis characterized by chromatic condensation and the loss of mitochondrial membrane potential in HT22 cells. In the in vivo study, TTC (2,3,5-triphenyltetrazolium chloride) staining showed a marked ischemic injury in blood supply territory of the middle cerebral artery (MCA) such as the cerebral cortex and striatum. However, treatment with SMT significantly reduced infarcted volume. SMT increased marked survival of HT22 cells against glutamate-induced cytotoxicity in MTT assay.
Conclusion: These results suggest that water extract of SMT provides neuroprotection against ischemic or oxidative injury by inhibition of apoptotic cell death.
목차
서론
재료 및 방법
실험결과
고찰
결론
참고문헌
참고문헌 (40)
참고문헌 신청
1. [학술지(정기간행물)] - 강성돈 / 1999 / 뇌졸중의 역학적 동향에 대한 연구 / 대한신경외과학학회지 28 : 509 ~ 513
2. [단행본] - American Heart Association / 1994 / Heart and Stroke Facts Statistical Supplement / American Heart Association : 12 ~
3. [학술지(정기간행물)] - Kirino T / 1985 / Selective vulnerability of the hippocampus to ischemia-reversible and irreversible types of ischemic cell damage / Progress in Brain Res 63 : 39 ~ 58
4. [학술지(정기간행물)] - Kogure T / 1997 / Molecular and biochemical events within the brain subjected to cerebral ischemia / Clin Neurosci 4 : 179 ~ 183
5. [학술지(정기간행물)] - Gray JA / 1982 / Comprasion between the behavioral effects of septal and hippocampal lesions. A review / Neuroscience and biobehavioral Reviews 7 : 119 ~ 188
2. [단행본] - American Heart Association / 1994 / Heart and Stroke Facts Statistical Supplement / American Heart Association : 12 ~
3. [학술지(정기간행물)] - Kirino T / 1985 / Selective vulnerability of the hippocampus to ischemia-reversible and irreversible types of ischemic cell damage / Progress in Brain Res 63 : 39 ~ 58
4. [학술지(정기간행물)] - Kogure T / 1997 / Molecular and biochemical events within the brain subjected to cerebral ischemia / Clin Neurosci 4 : 179 ~ 183
5. [학술지(정기간행물)] - Gray JA / 1982 / Comprasion between the behavioral effects of septal and hippocampal lesions. A review / Neuroscience and biobehavioral Reviews 7 : 119 ~ 188
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UCI(KEPA) : I410-ECN-0101-2014-519-000284296