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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 117 - 123 (7page)
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초록· 키워드
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Polyphenol oxidase (PPO) was purified from fresh ginseng roots using acetone precipitation, carboxymethyl (CM)-Sepharose chromatography, and phenyl-Sepharose chromatography. Two isoenzymes (PPO 1 and PPO 2) were separated using an ionexchange column with CM-Sepharose. PPO 1 was purified up to 13.2-fold with a 22.6% yield. PPO 2 bound to CM-Sepharose, eluted with NaCl, and was purified up to 22.5-fold with a 17.4% yield. PPO 2 was further chromatographed on phenyl-Sepharose. The molecular weight of the purified PPO 2 from fresh ginseng was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was about 40 kDa. The optimum temperature and pH were 20°C and 7.0, respectively, using catechol as a substrate. Pyrogallol showed the highest substrate specificity. The effect of a PPO inhibitor showed that its activity increased slightly in the presence of a low concentration of citric acid. High concentrations of acidic compounds and sulfite agents significantly inhibited purified ginseng PPO 2.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (34)
참고문헌 신청
1. [학술지(정기간행물)] - Mayer AM. / 2006 / Polyphenol oxidases in plants and fungi: going places? A review / Phytochemistry 67 : 2318 ~ 2331
2. [학술지(정기간행물)] - Flurkey WH / 2008 / Proteolytic processing of polyphenol oxidase from plants and fungi / J Inorg Biochem 102 : 2160 ~ 2170
3. [학술지(정기간행물)] - Van Gelder CW / 1997 / Sequence and structural features of plant and fungal tyrosinases / Phytochemistry 45 : 1309 ~ 1323
4. [학술지(정기간행물)] - Yoruk R / 2003 / Physicochemical properties and function of plant polyphenol oxidase: a review / J Food Biochem 27 : 361 ~ 422
5. [학술지(정기간행물)] - Thipyapong P / 2007 / Functional analysis of polyphenol oxidases by antisense/sense technology / Molecules 12 : 1569 ~ 1595
2. [학술지(정기간행물)] - Flurkey WH / 2008 / Proteolytic processing of polyphenol oxidase from plants and fungi / J Inorg Biochem 102 : 2160 ~ 2170
3. [학술지(정기간행물)] - Van Gelder CW / 1997 / Sequence and structural features of plant and fungal tyrosinases / Phytochemistry 45 : 1309 ~ 1323
4. [학술지(정기간행물)] - Yoruk R / 2003 / Physicochemical properties and function of plant polyphenol oxidase: a review / J Food Biochem 27 : 361 ~ 422
5. [학술지(정기간행물)] - Thipyapong P / 2007 / Functional analysis of polyphenol oxidases by antisense/sense technology / Molecules 12 : 1569 ~ 1595
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UCI(KEPA) : I410-ECN-0101-2014-524-000316474