지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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A Bacillus sp. that produces fibrinolytic enzyme was isolated from Cheonggukjang, a traditional Korean soybean-fermented food. According to 16S rRNA gene base sequencing, the bacillus was identified as a variety of Bacillus subtilis, and named Bacillus subtilis IDCC 9204. Fibrinolytic enzyme NK-IL9204 was stable up to 60oC and within pH range of 5-10. Purified NK-IL9204 was detected through fibrin zymography. The molecular weight and isoelectric point of the enzyme were estimated to be 27.7 kDa and 6.7 by SDS-PAGE and 2D electrophoresis, respectively. Its amino acid sequence was similar to that of nattokinase (identities 99.5%) and different from that of nattokinase BPN (identities 86.4%). The plasma fibrinolytic activity of NK-IL9204 was measured by euglobulin clot lysis times (ECLT). The NKIL9204 was orally administered to SD rats for 3 weeks (1,000 FU/rat/day). The ECLT was significantly shortened by supplementation of NK-IL9204.
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참고문헌 (22)
참고문헌 신청
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Sasaki K / 1985 / Transport of 125I-labeled human high molecular weight urokinase across the intestinal tract in a dog model with stimulation of synthesis and/or release of plasminogen activators / Blood 66:69~75
Pennica D / 1983 / Cloning and expression of human tissue-type plasminogen activator cDNA in E / coli. Nature 301:214~221
Medved LV / 1966 / Domain structure, stability and interactions in streptokinase / Eur. J. Biochem 239:333~339
Lijnen HR / 1991 / On the mechanism of fibrin-specific plasminogen activation by staphylokinase / J. Biol. Chem 266:11826~11832
Sasaki K / 1985 / Transport of 125I-labeled human high molecular weight urokinase across the intestinal tract in a dog model with stimulation of synthesis and/or release of plasminogen activators / Blood 66:69~75
Pennica D / 1983 / Cloning and expression of human tissue-type plasminogen activator cDNA in E / coli. Nature 301:214~221
Medved LV / 1966 / Domain structure, stability and interactions in streptokinase / Eur. J. Biochem 239:333~339
Lijnen HR / 1991 / On the mechanism of fibrin-specific plasminogen activation by staphylokinase / J. Biol. Chem 266:11826~11832
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UCI(KEPA) : I410-ECN-0101-2014-573-001113054