지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.7
- 수록면
- 8 - 12 (5page)
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초록· 키워드
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Purpose: A high yield of viable pancreatic islets is an essential prerequisite for the study of pancreatic islet transplantation. The purpose of this study is to compare the yield between intra-gall bladder (intra-GB) and intra-common bile duct (intra-CBD) injection of collagenase solution for isolation of mouse pancreatic islets.
Methods: The mice were divided into two groups, the intra-GB and intra-CBD groups, and each group included twelve mice, respectively. Collagenase solution was injected via the gallbladder in the intra-GB group mice, while this was done via the common bile duct in the intra-CBD group. After removal and digestion of the mouse pancreases, the pancreatic islets were isolated by Ficoll density gradient centrifugation and hand picking.
Results: The intra-GB group yielded 121.67±39.86 IEQs, and the intra-CBD group reveled 168.17±29.23 IEQs. There was a statistically significant difference in islet yield between the two groups (P=0.005, Mann-Whitney Test). The purities of the isolated islets were 86.42±3.99% for the intra-GB group and 87.17±4.47% for the intra-CBD group, and there was no difference between the two groups (P=0.755, Mann-Whitney Test).
Conclusion: Both the intra-GB and intra-CBD groups yielded an average of ＞120 IEQs. However, the intra-CBD group revealed a higher yield than the intra-GB group for isolating mouse pancreatic islets.
Methods: The mice were divided into two groups, the intra-GB and intra-CBD groups, and each group included twelve mice, respectively. Collagenase solution was injected via the gallbladder in the intra-GB group mice, while this was done via the common bile duct in the intra-CBD group. After removal and digestion of the mouse pancreases, the pancreatic islets were isolated by Ficoll density gradient centrifugation and hand picking.
Results: The intra-GB group yielded 121.67±39.86 IEQs, and the intra-CBD group reveled 168.17±29.23 IEQs. There was a statistically significant difference in islet yield between the two groups (P=0.005, Mann-Whitney Test). The purities of the isolated islets were 86.42±3.99% for the intra-GB group and 87.17±4.47% for the intra-CBD group, and there was no difference between the two groups (P=0.755, Mann-Whitney Test).
Conclusion: Both the intra-GB and intra-CBD groups yielded an average of ＞120 IEQs. However, the intra-CBD group revealed a higher yield than the intra-GB group for isolating mouse pancreatic islets.
목차
서론
방법
결과
고찰
결론
REFERENCES
참고문헌 (20)
참고문헌 신청
1. [학술지(정기간행물)] - Gurol AO / 2004 / A modified automated method for isolation of viable pancreatic islets in laboratory animals / Transplant Proc 36 : 1526 ~ 1527
2. [학술지(정기간행물)] - Lakey JR / 1997 / Development of an automated computer-controlled islet isolation system / Transplant Proc 29 : 1956 ~
3. [학술지(정기간행물)] - Yonekawa Y / 2006 / A new mouse model for intraportal islet transplantation with limited hepatic lobe as a graft site / Transplantation 82 : 712 ~ 715
4. [학술지(정기간행물)] - Osorio RW / 1994 / Prolongation of in vivo mouse islet allograft survival by modulation of MHC class I antigen / Transplantation 57 : 783 ~ 788
5. [학술지(정기간행물)] - Simeonovic CJ / 1986 / Survival and function of islet allografts in outbred mice / Transplant Proc 18 : 316 ~ 318
2. [학술지(정기간행물)] - Lakey JR / 1997 / Development of an automated computer-controlled islet isolation system / Transplant Proc 29 : 1956 ~
3. [학술지(정기간행물)] - Yonekawa Y / 2006 / A new mouse model for intraportal islet transplantation with limited hepatic lobe as a graft site / Transplantation 82 : 712 ~ 715
4. [학술지(정기간행물)] - Osorio RW / 1994 / Prolongation of in vivo mouse islet allograft survival by modulation of MHC class I antigen / Transplantation 57 : 783 ~ 788
5. [학술지(정기간행물)] - Simeonovic CJ / 1986 / Survival and function of islet allografts in outbred mice / Transplant Proc 18 : 316 ~ 318
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UCI(KEPA) : I410-ECN-0101-2013-514-003555920