지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Newcastle disease virus (NDV) Kr005/V strain was generated through 55 serial passages of NDV Kr005 strain in Vero cells. The Kr005/V virus yielded high infective titers of 107.8 TCID50/mL in Vero cells and the infected cells showed cytopathic effects such as marked cell rounding, though less frequent syncytia. The Kr005/V virus was heat-stable and classified into the lentogenic type with a Mean Death Time (MDT) of 120h or greater while the Kr005 strain was heat-labile and velogenic (MDT of 49.6 h). Only the single amino acid substitution (T to S) was observed at position 433 of the HN protein of the Kr005/V strain, whereas no amino acid change was found in the F protein. The Kr005/V input virus correlated well (correlation coefficient r2=0.97) with the Kr005 virus when ten field sera were tested by virus neutralization test. The biological properties and usefulness of Vero cell-adapted Kr005/V virus were discussed.
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참고문헌 (29)
참고문헌 신청
1. [학술지(정기간행물)] - Ahamed T / 2004 / Adaptation of Newcastle disease virus (NDV) on Vero cell line / Int J Poult Sci 3 : 153 ~ 156
2. [단행본] - Newcastle Disease Diagnosis / 1988 / Newcastle Disease Diagnosis. pp 147-160. In : Newcastle Disease, In Newcastle Disease / Kluwer Academic Publishers : 147 ~ 160
3. [단행본] - Alexander DJ / 2003 / Newcastle Disease, In Diseases of Poultry, 11th edition / Iowa State University Press : 64 ~ 87
4. [단행본] - Bankowski RA / 1964 / Propagation of Newcastle Disease Virus, In ewcastle Disease Virus: An Evolving Pathogen / University Wisconsin Press : 231 ~ 246
5. [학술지(정기간행물)] - de Leeuw OS / 2005 / Virulence of Newcastle disease virus is determinedby the cleavage site of the fusion protein and by both thestem region and globular head of the haemagglutinin-neuraminidaseprotein / J Gen Virol 86 : 1759 ~ 1769
2. [단행본] - Newcastle Disease Diagnosis / 1988 / Newcastle Disease Diagnosis. pp 147-160. In : Newcastle Disease, In Newcastle Disease / Kluwer Academic Publishers : 147 ~ 160
3. [단행본] - Alexander DJ / 2003 / Newcastle Disease, In Diseases of Poultry, 11th edition / Iowa State University Press : 64 ~ 87
4. [단행본] - Bankowski RA / 1964 / Propagation of Newcastle Disease Virus, In ewcastle Disease Virus: An Evolving Pathogen / University Wisconsin Press : 231 ~ 246
5. [학술지(정기간행물)] - de Leeuw OS / 2005 / Virulence of Newcastle disease virus is determinedby the cleavage site of the fusion protein and by both thestem region and globular head of the haemagglutinin-neuraminidaseprotein / J Gen Virol 86 : 1759 ~ 1769
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UCI(KEPA) : I410-ECN-0101-2013-527-002670658