지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.6
- 수록면
- 337 - 347 (11page)
이용수
초록· 키워드
오류제보하기
Purpose: In a previous study, we have shown that anticancer agents inhibiting topoisomerases improve survival of tumor cells under hypoxic condition. In the present study, we evaluated whether and how cell survival effect of the anticancer agents under hypoxic conditions could be eliminated by the addition of nitroimidazoles, a class of bioreductive agents.
Methods: Human hepatocellular carcinoma cells (HepG2) were incubated with different combinations of pimonidazole(1∼1,000μg/ml) and doxorubicin (0.1 or 1μg/ml) concentrations under different O₂ concentrations [1, 3, 5, 10 and 21 O₂]. Then cell numbers, glucose concentrations and lactic acid concentrations in the medium were measured, and DNA fragmentation assay was performed. Finally, different combinations of nitroimidazoles, such as pimonidazole, misonidazole, etanidazole, tinidazole, metronidazole, ornidazole or dimetridazole, and anticancer agents, such as doxorubicin, campothecin, epirubicin, dactinomycin, etoposide or mitomycin C was added to the cell culture medium under hypoxic conditions (1% O₂).
Results: Pimonidazole at a concentration of 100μg/ml eliminated cell survival effect of doxorubicin at the concentrations of 0.1 and 1μg/ml under hypoxic condition (1% O₂) by promoting apoptosis. Almost all the cells died even after 24 hours of incubation for all the oxygen concentrations at a combination of 100μg/ml pimonidazole and 1μg/ml doxorubicin. Finally, pimonidazole at a concentration of 100μg/ml, and misonidazole or etanidazole at a concentration of 1,000μg/ml eliminated cell survival effect of all the anticancer agents tested under hypoxic condition.
Conclusion: Combination therapy of doxorubicin (adriamycin) with pimonidazole can maximize dororubicin efficacy by eliminating cell survival effect of doxorubicin under hypoxic conditions in treating solid tumors, such as breast cancer.
Methods: Human hepatocellular carcinoma cells (HepG2) were incubated with different combinations of pimonidazole(1∼1,000μg/ml) and doxorubicin (0.1 or 1μg/ml) concentrations under different O₂ concentrations [1, 3, 5, 10 and 21 O₂]. Then cell numbers, glucose concentrations and lactic acid concentrations in the medium were measured, and DNA fragmentation assay was performed. Finally, different combinations of nitroimidazoles, such as pimonidazole, misonidazole, etanidazole, tinidazole, metronidazole, ornidazole or dimetridazole, and anticancer agents, such as doxorubicin, campothecin, epirubicin, dactinomycin, etoposide or mitomycin C was added to the cell culture medium under hypoxic conditions (1% O₂).
Results: Pimonidazole at a concentration of 100μg/ml eliminated cell survival effect of doxorubicin at the concentrations of 0.1 and 1μg/ml under hypoxic condition (1% O₂) by promoting apoptosis. Almost all the cells died even after 24 hours of incubation for all the oxygen concentrations at a combination of 100μg/ml pimonidazole and 1μg/ml doxorubicin. Finally, pimonidazole at a concentration of 100μg/ml, and misonidazole or etanidazole at a concentration of 1,000μg/ml eliminated cell survival effect of all the anticancer agents tested under hypoxic condition.
Conclusion: Combination therapy of doxorubicin (adriamycin) with pimonidazole can maximize dororubicin efficacy by eliminating cell survival effect of doxorubicin under hypoxic conditions in treating solid tumors, such as breast cancer.
목차
서론
방법
결과
고찰
결론
감사의 글
REFERENCES
참고문헌 (28)
참고문헌 신청
1. [학술지(정기간행물)] - Brahimi-Horn MC / 2007 / Hypoxia and cancer / J Mol Med 85 : 1301 ~ 1307
2. [학술지(정기간행물)] - Vaupel P / 2004 / Tumor hypoxia: causative factors, compensatory mechanisms, and cellular response / Oncologist 9 (5) : 4 ~ 9
3. [학술지(정기간행물)] - Wouters A / 2007 / Review: implications of in vitro research on the effect of radiotherapy and chemotherapy under hypoxic conditions / Oncologist 12 : 690 ~ 712
4. [학술지(정기간행물)] - Tannock IF / 2002 / Limited penetration of anticancer drugs through tumor tissue: a potential cause of resistance of solid tumors to chemotherapy / Clin Cancer Res 8 : 878 ~ 884
5. [학술지(정기간행물)] - Vaupel P / 2007 / Hypoxia in cancer: significance and impact on clinical outcome / Cancer Metastasis Rev 26 : 225 ~ 239
2. [학술지(정기간행물)] - Vaupel P / 2004 / Tumor hypoxia: causative factors, compensatory mechanisms, and cellular response / Oncologist 9 (5) : 4 ~ 9
3. [학술지(정기간행물)] - Wouters A / 2007 / Review: implications of in vitro research on the effect of radiotherapy and chemotherapy under hypoxic conditions / Oncologist 12 : 690 ~ 712
4. [학술지(정기간행물)] - Tannock IF / 2002 / Limited penetration of anticancer drugs through tumor tissue: a potential cause of resistance of solid tumors to chemotherapy / Clin Cancer Res 8 : 878 ~ 884
5. [학술지(정기간행물)] - Vaupel P / 2007 / Hypoxia in cancer: significance and impact on clinical outcome / Cancer Metastasis Rev 26 : 225 ~ 239
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