지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
RAD53 functions as an effector kinase of checkpoint pathways in Saccharomyces cerevisiae, which plays a central role to regulate many downstream cellular processes in response to DNA damage. It also involves in transcriptional activation of various genes including RNR genes which encode the key enzyme required for dNTP synthesis. In this study, we identified CYC8 as a suppressor for the hydroxyurea sensitivity of rad53Δ mutation. Rad53Δ mutant transformed with a multi-copy plasmid containing CYC8 showed increased hydroxyurea resistance. In contrast, TUP1 which forms a complex with CYC8 did not function as a suppressor. In the case of mutations, both cyc8Δ and tup1Δ suppressed hydroxyurea sensitivity of rad53Δ . Since CYC8 can propagate as a prion in yeast, overexpression of CYC8 induced misfolding of the normal CYC8 proteins, resulting in dominant cyc8 phenotype. Therefore, it is suggested that CYC8 can act as a multi-copy suppressor due to its prion property. It was observed that the levels of RNR transcription were increased in the yeast strains containing either multi-copies of CYC8 gene or cyc8Δ mutation, suggesting that the increased level of RNR will elevate the intracellular pools of dNTPs, which, in turn, suppress the phenotype of rad53Δ mutation.
목차
재료 및 방법
결과 및 고찰
적요
감사의 말
참고문헌
참고문헌 (21)
참고문헌 신청
1. [학술지(정기간행물)] - Baudin, A. / 1993 / A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae / Nucleic Acids Res 21 : 2239 ~ 3330
2. [학술지(정기간행물)] - Ben-Yehoyada, M. / 2007 / The DNA damage response during an unperturbed S-phase / DNA Repair 6 : 914 ~ 922
3. [학술지(정기간행물)] - Branzei, D. / 2005 / The DNA damage response during DNA replication / Curr. Opin. Cell. Biol 17 : 568 ~ 575
4. [학술지(정기간행물)] - Branzei, D. / 2006 / The Rad53 signal transduction pathway: replication fork stabilization, DNA repair, and adaptation / Exp. Cell Res 312 : 2654 ~ 2659
5. [학술지(정기간행물)] - Chabes, A. / 2003 / Survival of DNA damage in yeast directly depends on increased dNTP levels allowed by relaxed feedback inhibition of ribonucleotide reductase / Cell 112 : 391 ~ 401
2. [학술지(정기간행물)] - Ben-Yehoyada, M. / 2007 / The DNA damage response during an unperturbed S-phase / DNA Repair 6 : 914 ~ 922
3. [학술지(정기간행물)] - Branzei, D. / 2005 / The DNA damage response during DNA replication / Curr. Opin. Cell. Biol 17 : 568 ~ 575
4. [학술지(정기간행물)] - Branzei, D. / 2006 / The Rad53 signal transduction pathway: replication fork stabilization, DNA repair, and adaptation / Exp. Cell Res 312 : 2654 ~ 2659
5. [학술지(정기간행물)] - Chabes, A. / 2003 / Survival of DNA damage in yeast directly depends on increased dNTP levels allowed by relaxed feedback inhibition of ribonucleotide reductase / Cell 112 : 391 ~ 401
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UCI(KEPA) : I410-ECN-0101-2013-475-001685054