Propolis is a resinous substance honeybees forage from the buds and exudates of certain trees and plants which is then stored inside the beehives. Jeju is a southern island of Korea with a subtropical climate. The aim of this study is to examine the amount of total polyphenol and flavonoid contents and the antioxidant activity of the ethanol extracts of propolis (EEP) from Jeju Island. EEP were evaluated for antioxidant activities by β-carotene bleaching, 1,1-diphenyl-2-picylhydrazyl (DPPH) free radicalscavenging, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization, ferric reducing ability of plasma (FRAP) and inhibiting ability of soybean oil assays. Furthermore, we investigated the antibacterial and antifungal activities of propolis collected on Jeju Island. As a result, the amount of total polyphenol and flavonoid contents in Jeju Island propolis varied widely, ranging from 59.7 to 126.8mg/g of EEP and from 42.1 to 108.8mg/g of EEP, respectively. EEP samples were o bserved fifteen flavonoid and phenolic acid bands on high performance thin-layer chromatography (HPTLC) plate. Propolis from Bukjeju Nohyeung (J2) had relatively strong antioxidant activity accompanied by high total polyphenol, but that from Bukjeju Hallim (J5) had weak that. All EEP samples were more effective against gram-positive bateria than gram-negative ones and molds.