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논문 기본 정보

자료유형
학술저널
저자정보
Mi-Kyeong Kim (국립농업과학원) Hae-Ryun Kwak (국립농업과학원) Su-Heon Lee (국립농업과학원) Jeong-Soo Kim (국립농업과학원) Kook-Hyung Kim (서울대학교) ByeongJin Cha (충북대학교) Hong-Soo Choi (국립농업과학원)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.27 No.4
발행연도
2011.12
수록면
372 - 377 (6page)

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초록· 키워드

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A virus causing mottle and stunt symptom on Zea mays was observed around Ulleng-do, Korea and identified as Cucumber mosaic virus (CMV-ZM) based upon bio¬logical, serological, and molecular characteristics. In host range studies, the CMV-ZM isolate produced local lesions on Datura stramonium, Vigna unguiculata, Cucurbita moschata, Chenopodium amaranticolor, Ch. quinoa, whereas this isolate produced systemic mosaic on Nicotiana tabacum cv. ‘Xanthi-nc’, Capsicum annuum, Solanum Iycopersicum, Solanum melongena, Cucurbita pepo, and Z. mays. In addition, chlorotic local rings on inoculated leaves along with severe mosaic, malformation, and fern leaf symptoms on upper systemic leaves were shown in N. glutinosa plants. Complete nucleotide sequences of each genomic RNA segment was determined and compared to those of the other CMV strains. Comparison of the nucleotide sequence of 1a open reading frame (ORF) revealed approximately 89.2-92.4% sequence identity with each CMV subgroup IA and IB strain, while showing only 78% sequence identity with CMV subgroup Ⅱ. Nucleotide sequence analysis of RNA2 ORFs revealed 85.3-97.6% sequence identity with subgroup Ⅰ. In ORFs of RNA3, levels of nucleotide sequence identities were higher than 92¬99.2% with CMV subgroup Ⅰ and lower than 82% with CMV isolates of subgroup Ⅱ. These results suggest that CMV-ZM isolate is more closely related to subgroup Ⅰ than subgroup Ⅱ and therefore, CMV-ZM isolate might be classified into as CMV subgroup Ⅰ based on biological and molecular analysis.

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UCI(KEPA) : I410-ECN-0101-2013-481-001446131