지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Serotonin (5-hydroxytryptamine (5-HT))는 신경계의 세포-세포 간의 신호전달의 주요한 신경전달물질이다. 세포막에 존재하는 serotonin transporter (SERT)는 연접간격에 존재하는 5-HT를 세포 내로 재흡수 하여 세포외부의 5-HT 농도를 조절하지만 그 기전은 아직 밝혀지지 않았다. 본 연구에서는 yeast two-hybrid system을 사용하여 SERT의 C-말단이 protein kinase C-ε (PKC-ε)과 특이적으로 결합함을 알았다. PKC-ε는 PKC의 isotype으로 calcium 비의존적이며 phorbol ester/diacylglycerol 민감성 serine/threonine kinase이다. Na+/Cl- 의존성 SLC6 gene family의 다른 수송체는 PKC-ε과 결합하지 않았다. Deletion mutant들을 사용하여 SERT는 PKC-ε의 C-말단부위와 결합함을 알았으며, 또한 이 단백질간의 결합을 GST pull-down assay로 확인하였다. PKC-ε는 in vitro에서 SERT의 N-말단의 펩티드를 인산화시켰다. 이러한 결과들은 PKC-ε에 의한 SERT의 인산화가 세포막에 존재하는 SERT의 활성을 조절하는 역할을 할 가능성을 시사한다.
목차
Introduction
Materials and Methods
Results
Discussion
References
초록
참고문헌 (36)
참고문헌 신청
1. [학술지(정기간행물)] - Akita, Y / 2002 / Protein kinase C-epsilon (PKC-epsilon): its unique structure and function / J. Biochem 132 : 847 ~ 852
2. [학술지(정기간행물)] - Amara, S. G. / 1993 / Neurotransmitter transporters: recent progress / Annu. Rev. Neurosci 16 : 73 ~ 93
3. [학술지(정기간행물)] - Anderson, G. M / 1992 / Activators of protein kinase C decrease serotonin transport in human platelets / Biochim. Biophys. Acta 1137 : 331 ~ 337
4. [학술지(정기간행물)] - Barker, E. L. / 1998 / High affinity recognition of serotonin transporter antagonists defined by species-scanning mutagenesis. An aromatic residue in transmembrane domain I dictates species-selective recognition of citalopram and mazindol / J. Biol. Chem 273 : 19459 ~ 19468
5. [학술지(정기간행물)] - Benmansour, S. / 1999 / Effects of chronic antidepressant treatments on serotonin transporter function, density, and mRNA level / J. Neurosci : 10494 ~ 10501
2. [학술지(정기간행물)] - Amara, S. G. / 1993 / Neurotransmitter transporters: recent progress / Annu. Rev. Neurosci 16 : 73 ~ 93
3. [학술지(정기간행물)] - Anderson, G. M / 1992 / Activators of protein kinase C decrease serotonin transport in human platelets / Biochim. Biophys. Acta 1137 : 331 ~ 337
4. [학술지(정기간행물)] - Barker, E. L. / 1998 / High affinity recognition of serotonin transporter antagonists defined by species-scanning mutagenesis. An aromatic residue in transmembrane domain I dictates species-selective recognition of citalopram and mazindol / J. Biol. Chem 273 : 19459 ~ 19468
5. [학술지(정기간행물)] - Benmansour, S. / 1999 / Effects of chronic antidepressant treatments on serotonin transporter function, density, and mRNA level / J. Neurosci : 10494 ~ 10501
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UCI(KEPA) : I410-ECN-0101-2012-470-003590173