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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.50 No.4
- 발행연도
- 2009.8
- 수록면
- 334 - 340 (7page)
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초록· 키워드
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Twenty-six accessions were assessed for genetic diversity and phylogenetic relationships among Pyrus species by Inter-Simple Sequence Repeat (ISSR) markers. Among 45 ISSR primers used, only 16 ones were suitable for pear genomic DNA analysis and the range of polymorphic band rate was 55.6%-100% by primers. The 16 primers generated 173 markers, and the fragments size ranged from 200 bp to 2400 bp. According to Nei (1987) molecular evolutionary genetics, the summary of generic variation statistics for all loci in different species or groups showed that observed number of alleles was 1.20-1.44, effective number of alleles was 1.14-1.25, Nei’s gene diversity was 0.08-0.15, Shannon’s information index was 0.12-0.23, number of polymorphic loci was 35-76, and rate of polymorphic loci was 20.23-43.93%. P. bretschneideri and Dangshan local variety group had the highest genetic identity and the closest genetic distance, followed by inter-species hybrid group and ‘Dangshansuli’ and its mutant group. Occidental pear (P. communis) and Oriental pear (P. bretschneideri, P. pyrifolia, P. sinkiangensis, and P. ussuriensis) had lower genetic identity and farther genetic distance. Occidental pear and Oriental pear showed less relationship on the dendrogram of pear species & groups based on Nei’s Unbiased Measures genetic distance and the method of unweighted pair group method with arithmetic average (UPGMA) clustering modified from Neighbor Procedure of Phylip Version 3.5. Hierarchical cluster analysis showed there was positive difference between the clusters when all assayed accessions were divided into eight ones at the threshold value of 0.507-0.560. It was postulated that ‘Dangshansuli’ pear would originate by inter-species hybridization. The standpoints that P. bretschneideri, P. pyrifolia, and P. ussuriensis had closer relationship, and that Occidental pear (P. communis) and Oriental pear (P. bretschneideri, P. pyrifolia, P. ussuriensis, and P. betilaefolia) had the farthest genetic distance have been supported by analysis of ISSR markers in this experiment.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
Literature Cited
참고문헌 (36)
참고문헌 신청
1. [학술지(정기간행물)] - Fang, D.Q / 1997 / Identification of closely related citrus cultivars with inter-simple sequence repeat markers / Theor. Appl. Genet 95 : 408 ~ 417
2. [학술지(정기간행물)] - Fang, D.Q / 1998 / Phylogenetic relationships among selected citrus germplasm accessions revealed by inter-simple sequence repeat (ISSR) markers / . Amer. Soc. Hort. Sci 123 : 612 ~ 617
3. [학술지(정기간행물)] - Feng C.J / 2005 / Genetic diversity revealed by ISSR markers in apricot / Journal of Hebei Agricultural University 28 : 52 ~ 56
4. [학술지(정기간행물)] - Goulao, L / 2001 / Molecular characterization of cultivars of apple (Malus × domestica Borkh.) using microsatellite (SSR and ISSR) markers / Euphytica 122 : 81 ~ 89
5. [학술대회논문] - Karp, A / 1997 / Molecular tools in plant genetic resources conservation: a guide to the technologies / IPGRI technical bulletin no 2.
2. [학술지(정기간행물)] - Fang, D.Q / 1998 / Phylogenetic relationships among selected citrus germplasm accessions revealed by inter-simple sequence repeat (ISSR) markers / . Amer. Soc. Hort. Sci 123 : 612 ~ 617
3. [학술지(정기간행물)] - Feng C.J / 2005 / Genetic diversity revealed by ISSR markers in apricot / Journal of Hebei Agricultural University 28 : 52 ~ 56
4. [학술지(정기간행물)] - Goulao, L / 2001 / Molecular characterization of cultivars of apple (Malus × domestica Borkh.) using microsatellite (SSR and ISSR) markers / Euphytica 122 : 81 ~ 89
5. [학술대회논문] - Karp, A / 1997 / Molecular tools in plant genetic resources conservation: a guide to the technologies / IPGRI technical bulletin no 2.
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UCI(KEPA) : I410-ECN-0101-2009-525-018686910