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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.50 No.4
발행연도
2009.8
수록면
295 - 299 (5page)

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We attempted to establish a micropropagation system to increase the propagation efficiency of the epiphytic orchid Neofinetia falcata Hu. Explant materials used were shoot apexes, new stocks, scapes, leaves, and root tips taken from field-grown plants. The shoot apex was the most successful organ for the establishment of aseptic cultures. However, the cut surfaces of explants exuded browning compounds into the medium. The browning was prevented and bud formation was improved by dark-conditioning for two or three weeks in a shoot apex culture. To establish a sustainable micropropagation system using plantlets produced from the shoot apex culture, we investigated effects of dark-preconditioning, low temperature, and ascorbic acid on browning and bud formation. Explants derived from dark-preconditioned plantlets formed multiple buds, and did not produce browning compounds. Low temperature and ascorbic acid also prevented browning, but decreased survival ratio and bud formation. Additional low temperature or ascorbic acid treatments in addition to darkpreconditioning did not increase the survival ratio or bud formation as compared with the dark-preconditioning alone. These results show that N. falcata can be micropropagated by a shoot apex culture with two to three weeks of a dark-preconditioning treatment, and by repeated cultures of plantlets derived from shoot apex culture.

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Abstract
Introduction
Materials and Methods
Results
Discussion
Literature Cited

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