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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.3
- 수록면
- 75 - 78 (4page)
이용수
초록· 키워드
오류제보하기
A novel genotyping method, the Amp-FTA method, has been developed by combining the advantages of the Ampdirect<SUP>®</SUP> Plus and FTA<SUP>®</SUP> technologies. This method allowed the direct PCR amplification of DNA from unpurified blood immobilized on the FTA<SUP>®</SUP> card. PCR templates were prepared only by punching out the discs from the FTA<SUP>®</SUP> card, which could lead to a foolproof way of genotyping. The PCR products obtained could be subjected to downstream analyses such as restriction enzyme treatment and direct sequencing. The Amp-FTA method was applicable to buccal swabs collected by a simple technique minimizing distress to animals. Therefore, the Amp-FTA method is ideal for the genotyping and genetic monitoring of laboratory animals.
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UCI(KEPA) : I410-ECN-0101-2009-510-019510765