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자료유형
학술대회자료
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한국실험동물학회 한국실험동물학회 학술발표대회 논문집 2005년 한국실험동물학회 춘계학술대회
발행연도
2005.6
수록면
294 - 303 (10page)

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The tongue is a part of organ that plays important roles in swallowing, taste the food, and speech in human. Anatomically, the tongue is divided into an oral part and a pharyngeal part by the terminal sulcus. The following four different types of tongue papillae, which are small organs, can be found on the dorsal surface of the tongue: the fungiform, circumvallate, foliate and filiform papillae. Interestingly, development of fungiform papillae showed the stereotyped pattern on the dorsal surface of the tongue in mice from embryonic day 12 (E12) to E17. The patterned distribution and developmental processes of the fungiform papillae suggest possible similarities between the fungiform papillae and other epithelial appendages, including teeth, feathers, and hairs, which develop via epithelial mesenchymal interaction. The histological results and ultrastructural observations showed the development of specific structures in the epithelium into fungiform papillae. Many signalling factors, including signalling molecules and ECM (Extracellular matrix) molecules such as Shh, Bmps, Fgfs, Iaminin, CAM (Cell adhesion molecule) and cytokeratins, might be involved in the morphogenesis of fungiform papillae during the early development. In order to obtain direct evidence of the epithelium and mesenchyme interaction during fungiform papillae morphogenesis, the formation of fungiform papillae like structure was examined after a recombination assay. It was conducted after the recombination assay at E12.5 and E13.5 for 2 days using an in vitro organ culture. From the recombination assay results, the E13.5 epithelial portion of the fungiform papillae could determine the position of the newly formed fungiform papillae with the epithelial signalling molecules. E13.5 was a critical stage for fungiform papillae morphogenesis. To understand the signalling regulations of fungiform papillae, expression patterns of Shh, Bmp-2 and connexin 43 were examined. The results showed that the spatio-temporal expression pattern of signalling molecules is of evidence for proper formation of fungiform papillae. Furthermore, antisense oligodeoxynucleotide (AS-ODN) connexin 43 was treated to analyze the developmental functions of gap junction and NOGGIN protein, which is an antagonist of Bmps, was treated during in vitro organ culture. The expression patterns of signalling molecules, Shh and Bmp-2 were altered by inhibition of gap junctions. Shh, which is known as a key molecule to determine spacing pattern, expression patterns were disturbed after the inhibition of Bmp-2 as well. These results revealed that pathway of the signalling molecules during fungiform papillae development. Fungiform papillae can be considered to be small epithelial appendages, which are formed with the specific patterned array via the epithelial mesenchymal interactions.

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