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초록· 키워드

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This study was conducted to assess the possibility of skin wrinkle model by consecutive topical squalene monohydroperoxide(Sq-OOH) treatment to the hairless mice. Squalene monohydroperoxide was produced from squalene by UVA irradiation. Consecutive topical application of squalene monohydroperoxide to the hairless mice skin twice a day for 4 weeks induced the skin wrinkles with clear, deep and fine appearance. Skin wrinkles are appeared to be cross patterns without any particular direction, whereas parallel pattern was induced in the chronic UVB irradiated skin. The chronic UVB irradiated skin model has been known the most common animal model for skin wrinkle test. Photoaged skin showed the increment of epidermal thickness, shortened and fractured collagen bundles, and loss of total dermal collagen. Although similar appearance was occurred as seen in UVB irradiated skin in topical Sq-OOH treatment model, however, Sq-OOH treated skin induced more apparent skin wrinkles and more increment of epidermal thickness than those of chronic DVB irradiated skin. Histopathologically, more prominent keratinocyte hypertrophy was seen in Sq-OOH treated skin, comparison with chronic UVB irradiated group in epidermis. In dermis, degree of characteristic findings of UVB irradiated skin such as the loss of polarity and fractured collagen bundle and elastosis were not severely observed, while slightly increase of inflammatory cells were seen, in Sq-OOH treated skin. These results indicated that skin wrinkles and histopathological changes induced by Sq-OOH are different in many aspects from those by UVB irradiation. However, despite of widely used, chronic UVB irradiated model was spent a lot of time to achieve cumulative effect on the skin. We suggested that Sq-OOH model is a useful model to test the anti-wrinkle effects, because of its short induction time and more clear wrinkle formation.

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Introduction
Materials and methods
Results
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UCI(KEPA) : I410-ECN-0101-2009-510-016364690