지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.9
- 수록면
- 267 - 271 (5page)
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초록· 키워드
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Genomic DNA fragmentation was induced in intestinal cell nuclei of Haemonchus contortus by treatment of fenbendazole anthelmintic. Elucidating mechanisms to induce DNA fragmentation by an ectopic treatment might identify a novel way to induce irreparable damage to parasites. Since enzymatic deoxyribonucleases (DNases) are common cellular mediators that produce DNA fragmentation, research has been investigated to identify DNases of H. contortus. Among DNases detected, the DNases secreted from H. contortus adult worms occurred at 34, 36, 37 and 38.5 kDa. This research was conducted to further fractionate DNases secreted from H. contortus adult worms using chromatographic methods in order to elucidate roles of the individual DNases. The chromatographic methods applied included phenyl Sepharose, diethylaminoethyl Sepharose, DNA cellulose and Concanavalin-A Sepharose. According to characteristics of columns, some DNases were resolved in different fractions. Among chromatographic methods used, the 37 kDa acidic DNase could be separated from other DNases using fractionation with DNA cellulose or diethylaminoethyl Sepharose. While DNases of 34, 36 and 38.5 kDa were eluted in the 0.1 M NaCI fraction of diethylaminoethyl column and in the 0.1 M NaCI fraction of DNA column, separation of each DNase was not successful by the methods applied. Thus, additional steps are required for isolation of DNases at 34, 36 and 38.5 kDa. The research has laid a foundation to investigate the role of individual DNases secreted from H. contortus adult worms in mediating DNA fragmentation.
목차
Materials and Methods
Results
Discussion
References
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UCI(KEPA) : I410-ECN-0101-2009-510-016363133