메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색
질문

논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.48 No.6
발행연도
2007.12
수록면
376 - 380 (5page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색
질문

초록· 키워드

오류제보하기
We have developed a simple protocol for in vitro mass propagation of Anoectochilus formosanus using an automated low cost bioreactor system by a two-step culture. In the first step nodal and shoot tip cultures in gelled based media, N?-benzyladenine (BA) at 2 mgㆍL?¹ produced more shoots and higher weight than 2-isopentanyladenine (2iP) or kinetin (Kin). The shoot tips were also cultured in 3 l balloon type bubble bioreactor (BTBB) with different aeration volumes. Shoot proliferation and growth was the most effective when supply volume was 0.06 vvm. However, shoots developed in the bioreactor showed slow growth as well as small leaves. In the second step, shoots were transferred to 3 L BTBB containing 0.75 L Hyponex (20-20-20) 1 gㆍL?¹ + (6.5-4.5-19) 1 gㆍL?¹ medium with 2 gㆍL?¹ peptone, 3% sucrose, 0.5 gㆍL?¹ granular activated charcoal and different culture condition for shoot elongation and rooting. Comparative studies between gelled and bioreactor culture (continuous immersion [with or without net] and temporary immersion in liquid media using ebb and flood) revealed that shoot length was more efficient in gelled culture. However, the growth of plantlets was also higher in continuous immersion (with net) bioreactor culture. The effect of inoculation density on shoot growth during bioreactor culture was studied and maximum response was recorded when there were 60-90 inoculums per bioreactor included. Regenerated shoots with well developed roots were successfully acclimatized and transferred to greenhouse.

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited

참고문헌 (15)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

이 논문과 함께 이용한 논문

최근 본 자료

전체보기

댓글(0)

0

UCI(KEPA) : I410-ECN-0101-2009-525-016251055