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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.47 No.4
- 발행연도
- 2006.8
- 수록면
- 159 - 164 (6page)
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초록· 키워드
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β-Carotene hydroxylase (β-CHX) is a key enzyme of the carotenoid biosynthetic pathway and catalyzes the hydroxylation of β-carotene's β-ring to produce xanthophylls, β-cryptoxanthin, zeaxanthin, violaxanthin, and neoxanthin. In this study, we have isolated and characterized a full-length cDNA clone encoding β-CHX from rape (Brassica napus cv. Halla). The cDNA clone showed high sequence homology with β-CHX genes from various plant species and encoded 304 amino acids with a predicted molecular weight of 33 kDa. The expression of β-CHX gene was examined by Northern blot analysis in different tissues. The transcript for β-CHX was detected in leaves, petals, and flower buds, showing the highest expression level in flower buds. The result of HPLC analysis revealed that petals and flower buds contained high levels of hydroxylated carotenoid forms such as violoxanthin and neoxanthin. These results suggest that the high expression of β-CHX gene in flowers and flower buds might be closely related with change of carotenoid composition in these tissues.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited
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UCI(KEPA) : I410-ECN-0101-2009-525-016949192