지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.45 No.1
- 발행연도
- 2004.2
- 수록면
- 49 - 54 (6page)
이용수
초록· 키워드
오류제보하기
A heterologous cDNA probe from Petunia hybrida was used to isolate dihydroflavonol-4-reductase (DFR)-encoding cDNA clone from Dianthus caryophyllus. Comparison of the coding region of this DFR cDNA sequence including the sequences of Zea mays, Anthirrinum majus, Petunia hybrida, Matthiola incana, Callistephus chinensis, and Rosa hybrido reveals a similarity higher than 75% at the amono acid level. The DFR transcript is G/C rich in monocotyledonous plants show a strong codon bias preferring codons with a G or C in the third position. The function of this nucleotide sequences were verified by comparison with amino acid sequences of the amino-terminus and tryptic peptides from purified plant enzyme, by Northern blotting with mRNA from wild type and mutant plants and by in vitro expression yealding an enzymatically active reductase. Northern blot analysis of the DFR wild-type and mutant lines showed thai the lack of DFR activity in the stable acyanic mutants is clearly caused by a transcriptional block of the DFR gene.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited
참고문헌 (18)
참고문헌 신청
[학술지(정기간행물)] - / 1989 / Flavonoid synthesis in Petunia hybrida Partial characterization of dihydroflavonol-4-reductase genes : 491 ~ 502
[학술지(정기간행물)] - / 1994 / Characterization of the gene encoding dihydroflavonol 4-reductase in tomato : 153 ~ 157
[학술지(정기간행물)] - / 1984 / Cloning and experssion of the gene for bacteriophage T7 RNA polymerase : 2035 ~ 81 2038
[학술지(정기간행물)] - / 1997 / Flower color : 259 ~ 294
[학술지(정기간행물)] - / 1983 / A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity 132 : 6 ~ 13
[학술지(정기간행물)] - / 1994 / Characterization of the gene encoding dihydroflavonol 4-reductase in tomato : 153 ~ 157
[학술지(정기간행물)] - / 1984 / Cloning and experssion of the gene for bacteriophage T7 RNA polymerase : 2035 ~ 81 2038
[학술지(정기간행물)] - / 1997 / Flower color : 259 ~ 294
[학술지(정기간행물)] - / 1983 / A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity 132 : 6 ~ 13
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UCI(KEPA) : I410-ECN-0101-2009-525-016947289