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자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.45 No.1
발행연도
2004.2
수록면
38 - 42 (5page)

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Shoot tips of grape rootstock '5BB' (selected at the Korean Fruit Research Institute) were cultured to obtain disease-free plantlets. The shoots were cut into single nodes (2 ㎝ with a leaf), and cultured in bioreactors with three different culture methods; continuous immersion (with or without air exchanges) and temporary immersion (with air exchanges). Shoot growth was greatly affected by air exchanges in bioreactors, showing 1.5 times higher growth under air exchanges compared to without air exchanges. After 5 weeks of bioreactor culture, plantlets were cut into single nodes (4 ㎝-long with a leaf), transferred to ex vitro and grown at different nutrient solution pHs and ECs. Compared to water supply, nutrient solution supply (1.6 to dSㆍm?¹) induced 3-6 times greater shoot length, shoot dry weight, root dry weight, and total leaf area. Results also indicated that certain ions cannot be absorbed if the nutrient solution becomes too acid or alkaline. Plant growth was significantly different according to the pH of the nutrient solution: Shoot length, fresh and dry weight, total leaf area, and CO₂ uptake were greatest at pH 6 but lowest at pH 4 and 8.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited

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