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자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.46 No.1
발행연도
2005.2
수록면
87 - 92 (6page)

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To regenerate plants from root segments of Pinellia ternata, an oriental medicinal plant, the effect of growth regulators on callus induction and plant regeneration was investigated from root explants derived from in vitro propagated tuberlets. Adventitious roots from tuberlets showed the highest initiation on Murashige and Skoog (MS) media containing 0.2-0.5 ㎎ㆍL?¹ 1-naphthaleneacetic acid (NAA), but elongation of normal root was achieved on MS basal medium without growth regulators. The highest frequency of callus induction was on MS media supplemented with 1.0 ㎎ㆍL?¹ 2,4-dichlorophenoxyacetic acid and 0.5 ㎎ㆍL?¹ N-6-benzyladenine (BA). For plant regeneration, when calli were transferred to the first media supplemented with 1-4 ㎎ㆍL?¹ BA, most calli became hyperhydric, probably due to higher BA concentrations. At 60 days after transferring to the second regeneration medium containing 1.0 ㎎ㆍL?¹ NAA and 0.25 ㎎ㆍL?¹ kinetin, the frequency of plant regeneration from hyperhydric callus (85-92%) was lower than that from normal callus (100%), according to higher level of BA. Regenerated plants were successfully acclimatized in plastic pots containing a mixture of vermiculite:perlite:leaf-mold (1:1:1), and their survival rate was high.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Literature Cited

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