지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.46 No.1
- 발행연도
- 2005.2
- 수록면
- 43 - 48 (6page)
이용수
초록· 키워드
오류제보하기
The fruit acidity is a very important characteristic to select promising seedlings in peach breeding programs. Bulked segregant analysis (BSA) with randomly amplified polymorphic DNA (RAPD) analysis was performed to detect markers linked to D gene using F₁ seedlings of low acid ‘Yumyeong’ × low acid ‘Baekhyang’. One RAPD marker coupled to D allele, designated as UBC423₂₁??, was identified from 578 decamer primers examined. In addition, BSA with amplified fragment length polymorphism (AFLP) analysis of this progeny was performed to detect markers tightly linked to D gene. One AFLP marker, EAA/MCAC₃₃?, was identified through the 168 primer combinations tested. The co-segregation of two markers and D/d locus was verified in the 96 individuals of the F₁ progeny of ‘Yumyeong’ × ‘Baekhyang’. The markers UBC4232100 and EAA/MCAC₃₃? were found to be located at 22.1 and 1.1 cM, respectively, on the same side of the D/d locus. The tightly linked marker, EAA/MCAC₃₃?, was attempted to be converted to a sequence characterized amplified region (SCAR) marker. However, this marker could not be converted to SCAR marker. Although polymorphic SCAR could not be developed in the present study, the AFLP marker EAA/MCAC₃₃? could be useful for discriminating seedlings with high fruit acidity from peach seedlings at early developmental stage of the trees.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Literature Cited
참고문헌 (20)
참고문헌 신청
[학술지(정기간행물)] - / 1998 / Conversion of an AFLP fragment linked to the carrot Y2 locus to a simple, codominant, PCR-based marker form 97 : 960 ~ 967
[학술지(정기간행물)] - / 1998 / Genetic linkage map of peach [Prunus persica(L.) Batsch] using morphological and molecular markers 97 : 888 ~ 895
[학술지(정기간행물)] - / 1993 / Artifactual variation in randomly amplified polymorphic DNA banding patterns : 214 ~ 14 217
[학술지(정기간행물)] - / 2002 / Identification of RAPD and SCAR markers linked to the flesh adhesion gene F in peach(Prunus persica(L.) Batsch) 77 : 598 ~ 603
[학술지(정기간행물)] - / 1999 / Molecular linkage map construction and mapping genes for capscicinoids content and Bs3 in hot pepper
[학술지(정기간행물)] - / 1998 / Genetic linkage map of peach [Prunus persica(L.) Batsch] using morphological and molecular markers 97 : 888 ~ 895
[학술지(정기간행물)] - / 1993 / Artifactual variation in randomly amplified polymorphic DNA banding patterns : 214 ~ 14 217
[학술지(정기간행물)] - / 2002 / Identification of RAPD and SCAR markers linked to the flesh adhesion gene F in peach(Prunus persica(L.) Batsch) 77 : 598 ~ 603
[학술지(정기간행물)] - / 1999 / Molecular linkage map construction and mapping genes for capscicinoids content and Bs3 in hot pepper
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UCI(KEPA) : I410-ECN-0101-2009-525-016909150