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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.46 No.4
- 발행연도
- 2005.8
- 수록면
- 282 - 289 (8page)
이용수
초록· 키워드
오류제보하기
An efficient acclimatization of micropropagated Gymnaster koraiensis was developed through paclobutrazol pretreatment in vitro. Microshoots were induced on the MS medium containing a combination of 0.2 ㎎ㆍL?¹ thidiazuron and 0.1 ㎎ㆍL?¹ α-naphthaleneacetic acid, followed by culture on MS media containing combinations of 0.0?2.0 ㎎ㆍL?¹ paclobutrazol (PBZ) and 0.0?0.1 ㎎ㆍL?¹ indole-3-butyric acid (IBA) for 4 weeks. PBZ pretreatments in vitro at 0.5?1.0 ㎎ㆍL?¹ seemed to be more effective on the number of primary roots and fresh weight of root regardless of IBA concentration. Thus, PBZ pretreatment in vitro at 0.0, 0.5, 1.0, or 2.0 ㎎ㆍL?¹, respectively, was evaluated for in vitro root development and for acclimatization of plantlets in a greenhouse. Concerning in vitro rooting after 6 weeks of culture, root diameter and chlorophyll contents were increased in a PBZ concentration-dependent manner, whereas leaf area, leaf length, leaf shape index, and shoot and root length were markedly decreased. As PBZ concentration increased, stomatal index increased in adaxial leaf surface but it kept constant in abaxial surface. The plantlets obtained from each PBZ pretreatment in vitro were acclimatized in three commercial media under greenhouse condition. Soil mixture (peatmoss:perlite = 7:3, v/v) was the most suitable transplanting medium for acclimatization of this species regardless of in vitro PBZ concentration. The plantlets pretreated with PBZ at 0.5 ㎎ㆍL?¹ during in vitro culture showed the heavier fresh weight and dry weight than any other pretreated with PBZ concentrations. As a result, leaf morphology such as leaf shape index, stomatal index, and number of leaves seemed not to be significantly changed in PBZ-pretreated plantlets compared with normal plantlets after 16 weeks of acclimatization period. In conclusion, in vitro PBZ pretreatment at 0.5 ㎎ㆍL?¹ enhanced in vitro rooting and in vivo acclimatization of micropropagated G. koraiensis.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited
참고문헌 (17)
참고문헌 신청
[학술지(정기간행물)] - / 2005 / A general survey and some taxonomic implications of diterpenes in the Asteraceae 147 : 291 ~ 308
[학술지(정기간행물)] - / 2004 / Genetic relationships among Aster species by multivariate analysis and AFLP markers 140 : 193 ~ 200
[학술지(정기간행물)] - / 2001 / Screening of Aster germplasm for in vitro response to regeneration 55 : 255 ~ 260
[학술지(정기간행물)] - / 1991 / A taxonomic study on the genus Aster and its allied groups in Korea
[학술지(정기간행물)] - / 1997 / Digitalis obscura cardenolides: Effect of macronutrient concentration and N source on growth and productivity of shoot-tip cultures 46 : 273 ~ 278
[학술지(정기간행물)] - / 2004 / Genetic relationships among Aster species by multivariate analysis and AFLP markers 140 : 193 ~ 200
[학술지(정기간행물)] - / 2001 / Screening of Aster germplasm for in vitro response to regeneration 55 : 255 ~ 260
[학술지(정기간행물)] - / 1991 / A taxonomic study on the genus Aster and its allied groups in Korea
[학술지(정기간행물)] - / 1997 / Digitalis obscura cardenolides: Effect of macronutrient concentration and N source on growth and productivity of shoot-tip cultures 46 : 273 ~ 278
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UCI(KEPA) : I410-ECN-0101-2009-525-016908719