지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.12
- 수록면
- 419 - 425 (7page)
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초록· 키워드
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Phospholipase C-γ1 (PLC-γ1) is an important signaling molecule for cell proliferation and differentiation. PLC-γ1 contains two pleckstrin homology (PH) domains, which are responsible for protein-protein interaction and protein-lipid interaction. PLC-γ1 also has two Src homology (SH)2 domains and a SH3 domain, which are responsible for proteinprotein interaction. To identify proteins that specifically binds to PH domain of PLC-γ1, we prepared and incubated the glutathione S-transferase(GST)-fused PH domains of PLC-γ1 with COS7 cell lysate. We found that 90 kDa protein specifically binds to PH domain of PLC-γ1. By matrix-assisted laser desorption ionization time of flight-mass spectrometry, the 90 kDa protein revealed to be heat shock protein (Hsp) 90β. Hsp 90β is a molecular chaperone that stabilizes and facilitates the folding of proteins that are involved in cell signaling, including receptors for steroids hormones and a variety of protein kinases. To know whether Hsp 90β affects on PLC-γ1 activity, we performed PIP₂ hydrolyzing activity of PLC-γ1 in the presence of purified Hsp 90β in vitro. Our results show that the Hsp 90β dose-dependently inhibits the enzymatic activity of PLC-γ1 and further suggest that Hsp 90β regulates cell growth and differentiation via regulation of PLC-γ1 activity.
목차
서론
재료 및 방법
결과
고찰
REFERENCES
참고문헌 (29)
참고문헌 신청
1. [학술지(정기간행물)] - Berridge, MJ / 1993 / Inositol trisphosphate and calcium signaling / Nature 361 : 315 ~ 325
2. [학술지(정기간행물)] - Buchner, J / 1999 / Hsp90 & Co. - a holding for folding / Trends Biochem Sci. 24 : 136 ~ 141
3. [학술지(정기간행물)] - Bae, SS / 2000 / Proteolytic cleavage of phospholipase C-gamma1 during apoptosis in Molt-4 cells / FASEB J. 14 : 1083 ~ 1092
4. [학술지(정기간행물)] - Bai, XC / 2002 / Phospholipase C-gamma1 is required for survival in heat stress:involvement of protein kinase C-dependent Bcl-2 phosphorylation / J Biochem. 131 : 207 ~ 212
5. [학술지(정기간행물)] - Chang, JS / 2002 / Interaction of elongation factor-1alpha and pleckstrin homology domain of phospholipase C-gamma1 with activating its activity / J Biol Chem. 277
2. [학술지(정기간행물)] - Buchner, J / 1999 / Hsp90 & Co. - a holding for folding / Trends Biochem Sci. 24 : 136 ~ 141
3. [학술지(정기간행물)] - Bae, SS / 2000 / Proteolytic cleavage of phospholipase C-gamma1 during apoptosis in Molt-4 cells / FASEB J. 14 : 1083 ~ 1092
4. [학술지(정기간행물)] - Bai, XC / 2002 / Phospholipase C-gamma1 is required for survival in heat stress:involvement of protein kinase C-dependent Bcl-2 phosphorylation / J Biochem. 131 : 207 ~ 212
5. [학술지(정기간행물)] - Chang, JS / 2002 / Interaction of elongation factor-1alpha and pleckstrin homology domain of phospholipase C-gamma1 with activating its activity / J Biol Chem. 277
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UCI(KEPA) : I410-ECN-0101-2009-510-017674022