지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.9
- 수록면
- 177 - 183 (7page)
이용수
초록· 키워드
오류제보하기
To demonstrate the effect of formulation conditions and evaluations of structural integrity from ovalbumin containing poly lactide glycolide copolymer (PLGA) microspheres for Vaccine delivery, OVA microspheres were prepared by a W/O/W multiple emulsion solvent extraction technique. Dichloromethan (DCM) and Ethyl acetate (EA) were applied as an organic phase and poly vinyl alcohol (PVA) as a secondary emulsion stabilizer. Microspheres were characterized for particle size, morphology (optical microscopy and Scanning Electron Microscope (SEM)). Protein denaturation was evaluated by size exclusion chromatography (SEC), SDS-PAGE and isoelectric focusing (IEF). Residual organic solvent was estimated by gas chromatography (GC) and differential scanning calorimetry (DSC). Optical photomicrograph and SEM revealed that microspheres were typically spherical but various morphologies were observed. Mean particle size (dG<SUB>vs</SUB>) of microspheres were in the range of 3~50 μm. Also, The protein stability was not affected by the formulation process and residual organic solvent was beyond the detection below 0.1 ppm. These results demonstrated that microspheres might be a good candidate for the parenteral vaccine delivery system.
목차
영어초록
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (19)
참고문헌 신청
[학술지(정기간행물)] - T,Claudio / 1996 / Tetanus toxoid and synthetic malaria antigen containing poly(lactide)/poly(lactide-co-glycolide) microspheres.: importance of polymer degradation and antigen release for immune response / J Control Release 41 : 131 ~ 145
[단행본] - S,Cohen / 1996 / Microparticulate systems for the delivery of proteins and vaccines / Marcel Dekker, Inc. : 51 ~ 58
[단행본] - G,Crotts / 1995 / Preparation of porous and nonporous biodegradable polymeric hollow microspheres / J Control Rel 35 : 91 ~ 106
[학술지(정기간행물)] - BJA,Furr / 1987 / Pharmacological studies with zoladex, a novel luteinizing hormone-releasing hormone analogue(In Zoladex. A new treatment for prostatic cancer) / Royal society of Medicine International Congress and Symposium Series 125 : 1 ~ 10
[학술지(정기간행물)] - HS,Hora / 1990 / Release of human serum albumin from poly lactide-coglycolide microspheres / Pharm Res. 7 : 1190 ~ 1194
[단행본] - S,Cohen / 1996 / Microparticulate systems for the delivery of proteins and vaccines / Marcel Dekker, Inc. : 51 ~ 58
[단행본] - G,Crotts / 1995 / Preparation of porous and nonporous biodegradable polymeric hollow microspheres / J Control Rel 35 : 91 ~ 106
[학술지(정기간행물)] - BJA,Furr / 1987 / Pharmacological studies with zoladex, a novel luteinizing hormone-releasing hormone analogue(In Zoladex. A new treatment for prostatic cancer) / Royal society of Medicine International Congress and Symposium Series 125 : 1 ~ 10
[학술지(정기간행물)] - HS,Hora / 1990 / Release of human serum albumin from poly lactide-coglycolide microspheres / Pharm Res. 7 : 1190 ~ 1194
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UCI(KEPA) : I410-ECN-0101-2009-510-017357262