지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
α-Fetoprotein (AFP) is a good marker for the detection of several diseases such as hepatocellular carcinoma, gonadal germ cell tumor, gastric tumor, and Down's syndrome. In this study, we developed ELISA, using synthetic peptides corresponding to the epitopes of AFP. Five kinds of peptides were synthesized from AFP to produce antibodies in rats that recognize AFP in human plasma as well as amniotic fluid and do not cross-react with serum albumin. All five kinds of antibodies showed good reactivities with their peptide-keyhole limpet hemocyanin conjugates. Anti-synthetic peptide 1 (R-N-E-Y-G-I-A-S-I-L, 4-13) antibody, in particular, reacted well with AFP as well as synthetic peptide 1-KLH but not with human serum albumin. The binding affinity (Kd) was 2.7×l0?? M for peptide 1 and 6.8×10?? M for AFP. The range for measurement of AFP was 10~1,000 ng/㎖. The within-assay and between-assay coefficients of variance (CV) were 4.83% and 10.97%, respectively. In a sample of 31 sera and 33 amniotic fluids, there was a good correlation between AFP values determined in this assay and those in a commercial kit. These results indicate that the antibodies against synthetic peptides corresponding to the epitopes of AFP are highly specific to AFP and synthetic peptide-based ELISA would be useful for the measurement of human AFP.
목차
영어 초록
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
Acknowledgement
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Production of Polyclonal Antibody against α-Fetoprotein and Polyclonal Antibody-Based Competitive Enzyme-Linked Immunosorbent Assay for a-Fetoprotein
대한의생명과학회지
1997 .12
-
Persistent α-Fetoprotein Elevation in Healthy Adults and Mutational Analysis of α-Fetoprotein Promoter, Enhancer, and Silencer Regions
Gut and Liver
2017 .01
-
새로운 ARCHITECT AFP Assay의 분석능 평가
Laboratory Medicine Online
2018 .01
-
Prognostic Significance of Preoperative Serum Alpha-fetoprotein in Hepatocellular Carcinoma and Correlation with Clinicopathological Factors: a Single-center Experience from China
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Alpha-fetoprotein (AFP) Elevation Gastric Adenocarcinoma and Importance of AFP Change in Tumor Response Evaluation
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
수술 전 알파태아단백치가 높은 간세포암 환자에서 간절제술 후 알파태아단백만을 이용한 추적관찰이 가능한가?
Clinical and Molecular Hepatology
2010 .01
-
Distinctions Between Clinicopathological Factors and Prognosis of Alpha-fetoprotein Negative and Positive Hepatocelluar Carcinoma Patients
Asian Pacific journal of cancer prevention : APJCP
2012 .01
-
Production of a Monoclonal Antibody to Human a-Fetoprotein and Development of Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assays for Human α-Fetoprotein
대한의생명과학회지
1999 .06
-
정상 임산부의 혈청 Alpha-fetoprotein치의 임상적 이용
영남의대학술지
1997 .01
-
AFP 검사방법 단축에 관한 고찰
핵의학기술
2013 .01
-
Prognostic Value of Serum AFP, AFP-L3, and GP73 in Monitoring Short-term Treatment Response and Recurrence of Hepatocellular Carcinoma after Radiofrequency Ablation
Asian Pacific journal of cancer prevention : APJCP
2014 .01
-
Alpha feto protein(AFP)검사 키트의 유효성 평가실험
핵의학기술
2018 .01
-
알파태아단백을 분비하는 원발성 폐 선암 1예
Tuberculosis and Respiratory Diseases
2006 .01
-
The effects of newly formed synthetic peptide on bone regeneration in rat calvarial defects
Journal of Periodontal & Implant Science
2010 .01
-
B형 간염 보균자의 위험평가 수단으로서의 AFP 활용가치에 대한 고찰
보험의학회지
2006 .01
-
혈청 알파태아단백치가 현저히 상승한 대장암의 간전이 1예
Clinical and Molecular Hepatology
2006 .01
-
Alpha-fetoprotein 측정용 국산 방사면역측정법 시약의 평가
대한핵의학회지
2000 .01
-
방사면역측정법을 이용한 혈청 Alpha-fetoprotein치의 임상적 고찰
대한방사선의학회지
1982 .01
-
혈청 알파태아단백이 증가된 순수 미성숙 기형종 - 1예 보고 -
Journal of Pathology and Translational Medicine
2007 .01
-
알파 태아단백을 분비하는 원발성 폐암 1예
Tuberculosis and Respiratory Diseases
2012 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-510-017339715