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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Oligopeptide is known to be an essential nitrogen nutrient for bacterial growth. Oligopeptide can be transported into cytoplasm by a specific transport system, Opp system. Opp system is composed of five proteins, which are transcribed by an operon. These are responsible for oligopeptide binding protein (OppA), permease (OppB and OppC) and energy generation system (OppD and OppF), respectively. Previously, we isolated the opp operon from Vibrio fluvialis and constructed the oppA mutant by allelic exchange method. In this study, we investigated the growth pattern and biofilm production under the different growth condition. When the cells were cultivated using brain heart infusion(BHI) medium, the wild type was faster than the mutant in growth during the exponential phase. However, it showed that the growth pattern of two strains in M9 medium is very similar. The growth of wild type showed better than that of the mutant grown at pH 8. At pH 7, there was no an obvious difference in growth. After 5 mM H₂O₂was treated to the cells (OD_(600)=1.2), the cell survival was examined. The oppA mutation did not affect in survivability. In the presence of 10 ㎍/ml polymyxin B, the biofilm production of the oppA mutant was higher than that of the wild type.
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Abstract
재료 및 방법
결과 및 고찰
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감사의 글
참고문헌
참고문헌 (28)
참고문헌 신청
Borezee, E., E. Pellegrini and P. Berche. 2000. OppA of Listeria monocytogenes, an oligopeptide-binding protein required for bacterial growth at low temperature and involved in intracellular survival. Infect. Immun. 68, 7069-7077.
Cundell, D. R., B. J. Pearce, J. Samderls, A. M. Naughton and H. R. Masure. 1995. Peptide permease from Streptococcus pneumoniae affect adherence to eukaryotic cells. Infect. Immun. 63, 2493-2498.
Darmstadt, G. L., L. Mentele, A. Podbielski and C. E. Rubens. 2000. Role of group A Streptococcal virulence factors in adherence to keratinocytes. Infect. Immun. 68, 1215-1221.
Detmers, F. J. M., F. C. Lanfermeijer and B. Poolman, 2001. Peptides and ATP binding cassette peptide transporters. Res. Microbiol. 152, 245-258.
Goodell, E. W. and C. F. Higgins. 1987. Uptake of cell wall peptides by Salmonella typhimurium and Escherichia coli. J. Bacteriol. 169, 3861-3865.
Cundell, D. R., B. J. Pearce, J. Samderls, A. M. Naughton and H. R. Masure. 1995. Peptide permease from Streptococcus pneumoniae affect adherence to eukaryotic cells. Infect. Immun. 63, 2493-2498.
Darmstadt, G. L., L. Mentele, A. Podbielski and C. E. Rubens. 2000. Role of group A Streptococcal virulence factors in adherence to keratinocytes. Infect. Immun. 68, 1215-1221.
Detmers, F. J. M., F. C. Lanfermeijer and B. Poolman, 2001. Peptides and ATP binding cassette peptide transporters. Res. Microbiol. 152, 245-258.
Goodell, E. W. and C. F. Higgins. 1987. Uptake of cell wall peptides by Salmonella typhimurium and Escherichia coli. J. Bacteriol. 169, 3861-3865.
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UCI(KEPA) : I410-ECN-0101-2009-470-015376317