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학술대회자료
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한국가금학회 한국가금학회 심포지움 한국가금학회 2001년도 하계 Symposium Proceeding of Korean Sosiety of Poultry Science
발행연도
2001.8
수록면
19 - 26 (8page)

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The goal of this paper was to examine the quality zygote-acquiring method for in-vitro culture and the in-vitro culture method of the acquired zygote from a technological perspective. We have reported the results on the introduction of foreign DNAs using the described culturing method. After performing in-vitro and surrogate eggshell culture on a zygote acquired from the abdomen of a hen, 25.8% hatchability was acquired. After microinjecting foreign DNAs into the acquired zygote and performing in-vitro and surrogate eggshell culture using the same method, 13.1~11.7% hatchability was acquired. Having compared the developments of the control subjects and the experimental subjects, the viability of the experimental subjects on the 4~5th day of culturing was much lower compared to that of the control subjects. This is a result that shows that the microinjection process of foreign DNAs might have a negative effect on the existence of the embryo; therefore, various technical attempts should be made to minimize such negative effects. Having microinjected foreign DNAs into the zygote of a hen to produce transgenic chickens, 3 transgenic founders were produced and 70 G1 progeny were produced as a result of the progeny test that had been performed to the present.

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ABSTRACT

Introduction

Preparation of the Donor Hen

Preparation of the Surrogate Eggshell

Culturing the Zygote

Injection of Foreign DNA

Screening for Transgenic Birds

Viability and Hatchability of Cultured Embryos

Production of Transgenic Chickens

Conclusions

References

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