지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The biotin-avidin complex, as a model recognition system, has been constructed through N-hydroxysuccinimide (NHS) reaction on a variety of substrates such as a smooth Au film, electrochemically roughened Au electrode and chemically modified mica. Stepwise self-assembled monolayers (SAMs) of biotin-avidin system were characterized by surface-enhanced resonance Raman scattering (SERRS) spectroscopy, atomic force microscopy (AFM) and surface plasmon resonance (SPR). A strong SERRS signal of rhodamine tags labeled in avidin from the SAMs on a roughened gold electrode indicated the successful complex formation of stepwise biotin-avidin recognition system. AFM images showed the circular shaped avidin aggregates (hexamer) with ca. 60 Å thick on the substrate, corresponding to one layer of avidin. The surface coverage and concentration of avidin molecules were estimated to be 90% and 7.5×10-¹² mol/㎠, respectively. SPR technique allowed one to monitor the surface reaction of the specific recognition with high sensitivity and precision.
목차
Abstract
1. Introduction
2. Experimental
3. Results and Discussion
4. Conclusion
Acknowledgement
References
참고문헌 (20)
참고문헌 신청
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A. A. Karyakin, G. V. Presnova, M. Y. Rubtsova and A. M. Dgorov, Anal. Chem. 72, 3850(2000).
K. L. Prime and G. M. Whitesides, Science, 252, 1164(1991).
N. M. Green, Biochem. J., 89, 599(1963).
A. Ulman, “An Introduction to Ultrathin Organic Films from Langmuir-Blodgett to Self-assembly” Academic Press, New York (1991).
A. A. Karyakin, G. V. Presnova, M. Y. Rubtsova and A. M. Dgorov, Anal. Chem. 72, 3850(2000).
K. L. Prime and G. M. Whitesides, Science, 252, 1164(1991).
N. M. Green, Biochem. J., 89, 599(1963).
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