지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.3 No.1
- 발행연도
- 1998.3
- 수록면
- 98 - 104 (7page)
이용수
초록· 키워드
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As an effort to construct LAB(lactic acid bacteria), capable of utilizing starch as fermentation substrate without the aid of externally supplied enzymes, plasmid vectors containing the amyL(α-amylase gene) from Bacillus licheniformis, apu(α-amylase/pullulanase gene) from Clostridium thermohydrosulfuricum, and glucoamy-lase cDNA from Aspergillus shirousamii were constructed and introduced into E. coli and L. lactis. For expression in procaryotes, 1.9 kb glucoamylase cDNA encoding the mature form of enzyme was PCR amplified and translationally fused to a PCR amplified 260 bp fragment containing the promoter and secretion signals of amyL in the same reading frame. The production of α-amylase, Apu, and glucoamylase in E. coli and L. lactis was confirmed by enzyme assay and zymography. Enzymes were detected in both cell pellets and supernatants, indicating the working of secretion signals in heterologous hosts. The efficiencies of secretion were variable depending on the gene and host. The highest α-amylase activity observed was 1.1 units and most activity was detected from the cell pellets. The degree of gene expression in both hosts and the effect on the growth of hosts were examined.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
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UCI(KEPA) : I410-ECN-0101-2009-511-018074334