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자료유형
학술저널
저자정보
저널정보
한국독성학회 Toxicological Research Journal of Toxicology and Public Health Vol.15 No.2
발행연도
1999.6
수록면
199 - 204 (6page)

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We have studied, by utilizing a nonisotopic in situ DNA end-labeling (ISEL) technique, frequency of apoptosis in the intestinal crypt cell of adult mice exposed to y-ray irradiation from 60CO or diagnostic ultrasound. The extent of changes following 200 cGy (1090 cGy/min) was studied at 2, 4, 6, 8, 12, or 24 hours after exposure. The maximal frequency was found 4~6 hours after exposure. The mice that received 18, 36, 54, 108, 198, 396 cGy of y-rays or diagnostic ultrasound (7.5 MHz, 4.2 mW, ISPTA =7.9 mW/㎠, ISPPA= 114.3 W/㎠) for 10 or 30 minutes were examined at 6 hours after irradiation. Measurements performed after y-ray irradiation showed a dose-related increase in proportion of apoptotic cells in each of the mice studied. The dose-response curves were analyzed by a linear-quadratic model; frequency (number per crypt) of apoptotic cells in the intestinal crypt of adult mice was (0.0386±0.004204)D+(-0.0000535±0.00001120)D²+0.15475 (r²=0.952, D=dose in cGy). In the experiment of ultrasound exposure, the frequencies of apopototic cells were 0.181±0.055 (10 minutes exposure) and 0.225±0.137(30 minutes exposure). We estimated the relative dose of the yield from the experiment with ultrasound by substituting the yield from ultrasound exposure into the curve from the y-irradiation. The relative doses of ultrasound exposure compared with y-irradiation were 0.692 cGy (IO minutes exposure) and 1.334 cGy (30 minutes exposure).

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ABSTRACT

Ⅰ. 서론

Ⅱ. 재료 및 방법

Ⅲ. 결과

Ⅳ. 고찰

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