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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2003.6
- 수록면
- 133 - 139 (7page)
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Allergic contact dermatitis may be caused by a wide variety of chemicals. A murine local lymph node assay (LLNA) has been developed as an alternative to guinea pig models for assessing the contact sensitization potential of chemical. However, there is a need to develop a nonradioisotopic endpoint for the LLNA, because of the radioisotopic method's requiring the use of special
facilities. In this study, we investigated the development of a non-radioisotopic endpoint for LLNA using ELISA (enzyme-linked immunosorbent assay). Female Balb/c mice were treated by the topical application on the dorsum of both ears with four different strong sensitizers, 2,4-dinitrochlorobenzene (DNCB), oxazolone (OXZ), toluene diisocyanate (TDI), and trimellitic anhydride (TMA), and a strong irritant, sodium lauryl sulfate (SLS), once daily for three consecutive days. The proliferation of cells in the auricular lymph node was analyzed by means of the labelling index (LI) of bromodeoxyuridine (BrdU) incorporation into cells. The weights of the lymph nodes in the mice treated with allergens, DNCB, OXZ, TDI and TMA were increased compared to the vehicle control. The stimulation index (SI) of mice treated with DNCB, OXZ, TDI, and TMA was over three-fold increase compared to the vehicle control. However, the SI of mice exposed to SLS was not significantly increased compared to the vehicle control, while the lymph node weight of SLS was significantly increased. These results suggest that the LLNA modified endpoint using ELISA based on BrdU incorporation could provide a useful method of screening for irritants and allergens.
facilities. In this study, we investigated the development of a non-radioisotopic endpoint for LLNA using ELISA (enzyme-linked immunosorbent assay). Female Balb/c mice were treated by the topical application on the dorsum of both ears with four different strong sensitizers, 2,4-dinitrochlorobenzene (DNCB), oxazolone (OXZ), toluene diisocyanate (TDI), and trimellitic anhydride (TMA), and a strong irritant, sodium lauryl sulfate (SLS), once daily for three consecutive days. The proliferation of cells in the auricular lymph node was analyzed by means of the labelling index (LI) of bromodeoxyuridine (BrdU) incorporation into cells. The weights of the lymph nodes in the mice treated with allergens, DNCB, OXZ, TDI and TMA were increased compared to the vehicle control. The stimulation index (SI) of mice treated with DNCB, OXZ, TDI, and TMA was over three-fold increase compared to the vehicle control. However, the SI of mice exposed to SLS was not significantly increased compared to the vehicle control, while the lymph node weight of SLS was significantly increased. These results suggest that the LLNA modified endpoint using ELISA based on BrdU incorporation could provide a useful method of screening for irritants and allergens.
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UCI(KEPA) : I410-ECN-0101-2009-513-013746251