Propylthiouracil을 이용한 OECD enhanced TG407의 내분비계 장애 물질검색을 위한 유효화 실험

강경선; 김대용; 제정환; 김태원; 김형섭; 박지은; 윤준원; 김경배; 이지해; 조성대; 조종호; 최재훈; 이중근; 이영순

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자료유형: 학술저널

저자정보: 강경선 김대용 제정환 김태원 김형섭 박지은 윤준원 김경배 이지해 조성대 조종호 최재훈 이중근 이영순

저널정보: 한국독성학회 Toxicological Research Journal of Toxicology and Public Health Vol.17 No.3

발행연도: 2001.9

수록면: 203 - 213 (11page)

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We investigated the toxic effects of propylthiouracil (PTU) in Sprague-Dawley (SD) rats to develop and validate an enhanced protocol for Test Guideline 407 as OECD project. Twenty male and female SD rats, 7 weeks old, were treated with PTU in corn oil at levels of 0, 0.1, 1 and 10 mg/kg/day for 4 weeks orally. Clinical observation, body weight changes, food uptake, water consumption, urinalysis, estrus cycle and sperm analysis, serum chemistry, autopsy findings and histopathological findings were evaluated in this study. No clinical signs and mortality were observed in the study. The body weights and food uptakes in the group treated with 10 mg/kg/day were reduced from 3 weeks after the initiation of the treatment. The levels of 3,5,3''-triiodothyronine (T3) and thyroxine (T4, 3,5,3'',5''-tetraiodothyrosine) were also significantly decreased in the group treated with 10 mg/kg/day. Also, the relative and absolute organ weights of thymuses were decreased. Thyroid glands of rats in the group treated with PTU 10 mg/kg/day were bigger than those of rats in the control group. In the histopathological examination, diffuse hyperplasia and hypertrophy of thyroid follicular cells were observed in all treatment groups, leading to the reduction of lumen size and papillary enfolding of lining epithelium. The degree of lesion was increased in a dose-dependent manner. The results suggested that PTU would cause toxicity in thyrold gland and decrease the levels of T3 and T4 in SD rats. However, there were no effects on the other organs including testis and uterus especially in spermatogenesis and estrus cycle. On the basis of the results, enhanced protocol for Test Guideline (TG) 407 may be sensitive and reliable to detect endocrine-active substances like PTU.

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강경선

소속기관 Seoul National University

주요연구분야 의약학 > 수의학 의약학 > 기초의학 > 예방의학·직업환경의학

논문수 64 이용수 3,186

Dae Yong Kim

소속기관 서울대학교

주요연구분야 의약학 > 수의학 의약학 > 기초의학 > 예방의학·직업환경의학

논문수 33 이용수 612

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소속기관 한국독성학회