지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 전창진
- 발행연도
- 2023
- 저작권
- 경북대학교 논문은 저작권에 의해 보호받습니다.
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Purpose: The purpose of this study is to study the distribution pattern of cholinergic amacrine cells in the diurnal Mongolian gerbil retina.
Methods: The localization of choline acetyltransferase-immunoreactive(ChAT-IR) cells in the gerbil retina were investigated using immunocytochemistry and confocal microscopy. Cell density of ChAT-IR cells were quantitatively analyzed.
Results: ChAT-IR cells were present in the inner nuclear layer and in the ganglion cell layer, and the processes of these cells form two distinct narrow bands in the inner plexiform layer as previously reported in various animals. The average density of ChAT-IR cells in the ganglion cell layer was 1,242±111 cells/mm2 and 1,487±257 cells/mm2 in the inner nuclear layer showing that the number of ChAT-IR cells in the inner nuclear layer was higher than that of the ganglion cell layer. The cholinergic amacrine cells in the gerbil retina contained calcium-binding protein calretinin, but not calbindin-D28K or parvalbumin.
Conclusions: The distributional pattern show that cholinergic amacrine cells in the gerbil retina are similar to those of other animals, the results of the expression of calcium-binding proteins in cholinergic amacrine * A thesis submitted to the Council of the Graduate School of Kyungpook National University in partial fulfillment of the requirements for the degree of Master of Science in june 2023
cells are species-specific. The
cholinergic amacrine cells are known to play key roles in the motion detection mechanisms in retina, and thus the present study in gerbil retina might provide better information for further physiological studies of diurnal animal model.
Methods: The localization of choline acetyltransferase-immunoreactive(ChAT-IR) cells in the gerbil retina were investigated using immunocytochemistry and confocal microscopy. Cell density of ChAT-IR cells were quantitatively analyzed.
Results: ChAT-IR cells were present in the inner nuclear layer and in the ganglion cell layer, and the processes of these cells form two distinct narrow bands in the inner plexiform layer as previously reported in various animals. The average density of ChAT-IR cells in the ganglion cell layer was 1,242±111 cells/mm2 and 1,487±257 cells/mm2 in the inner nuclear layer showing that the number of ChAT-IR cells in the inner nuclear layer was higher than that of the ganglion cell layer. The cholinergic amacrine cells in the gerbil retina contained calcium-binding protein calretinin, but not calbindin-D28K or parvalbumin.
Conclusions: The distributional pattern show that cholinergic amacrine cells in the gerbil retina are similar to those of other animals, the results of the expression of calcium-binding proteins in cholinergic amacrine * A thesis submitted to the Council of the Graduate School of Kyungpook National University in partial fulfillment of the requirements for the degree of Master of Science in june 2023
cells are species-specific. The
cholinergic amacrine cells are known to play key roles in the motion detection mechanisms in retina, and thus the present study in gerbil retina might provide better information for further physiological studies of diurnal animal model.
목차
Ⅰ. 서론 1Ⅱ. 대상 및 방법 4Ⅱ-1. 대상 및 망막 조직 준비 4Ⅱ-2. 표준면역세포화학법 4Ⅱ-3. 정량 분석 5Ⅲ. 결과 6Ⅲ-1. ChAT-IR 세포의 분포 6Ⅲ-2. ChAT-IR 세포 및 칼슘결합단백질-IR 세포의 면역세포화학적 동정 10Ⅳ. 고찰 16Ⅴ. 결론 19Ⅶ. 참고문헌 20영문초록 29
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