Sewage & wastewater treatment plants are known to be major sources of the discharge of various micropollutants (e.g., pharmaceutical, pesticide, industrial chemical.) present in surface water. Micropollutants flowed into surface water are recognized as a risk to human health and aquatic ecosystem. This study attempted to quantitatively analyze micropollutants present in surface water through target screening of 314 species based on LC-HRMS and evaluate their toxic effects & bioaccumulation on Daphnia magna. Sample collection was performed near Jincheoncheon located in Daegu Metropolitan City, and the pre-treatment was applied with the SPE(Solid Phase Extraction) technique using a multi-layer cartridge. The analysis sample was concentrated (C1, C10, RQ1, and RQ10) at a certain ratio based on the concentration & RQ(Risk Quotient) to perform a D.magna chronic toxicity test. As a result of quantitative analysis for 314 targets, 109 substances were detected. Major substances(RQ>0.5) were identified through risk assessment based on quantitative analysis. RQ1 included 3 species(telmisartan, 2-acetamidophenol, galaxolidone) and RQ10 included 20 species(telmisartan, DIPEA, 2-acetamidophenol, olmesartan, galaxolidone, losartan, penconazole, sulfapyridine, crotamiton crs, TPPO, rac n-desmethyl tramadol, sulfamethoxazole, procaine, amisulpride, climbazole, 2-aminobenzothiazole, caffeine, 2-aminobenzimidazole, lamotrigine, TBEP). The results of the chronic toxicity test showed that only RQ10 had 20% fatality, and the growth rate of adult daphnia was also the lowest. Reproduction decreased to C1(83%), C10(73%), RQ1(80%), and RQ10(62%) compared to the control, and the number of dead youngs and abortive eggs increased significantly from C10. As a result of the bioaccumulation experiment, only galaxolidone was detected below LOQ in RQ10. These results suggest that micropollutants present in the surface water can negatively affect aquatic organisms such as D.magna, and it is expected that the substances affecting them may differ depending on the endpoint. In addition, it is judged that some substances with high RQ represent the overall risk of water quality.