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논문 기본 정보

자료유형
학위논문
저자정보

정유진 (동의대학교, 동의대학교 대학원)

지도교수
홍상훈
발행연도
2022
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동의대학교 논문은 저작권에 의해 보호받습니다.

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2022

오자연종환(五子衍宗丸) 추출물의 항산화 효과에 관한 연구
정유진 ,  박상은 ,  홍상훈 대한한방내과학회지 2022.06 학술저널
眞心有生丸(五子衍宗丸 加減方)추출물의 항산화 효과에 관한 연구
정유진 한의 2022.01 학위논문

초록· 키워드

오류제보하기
In this study, the antioxidant activity of Gagambang, Ojayeonjong-hwan, and
Jinsimyusaeng-hwan extracts was compared, and the following results were
obtained.
1. In comparison of DPPH free radical and ABTS cationic radical scavenging
activity, water and 70% ethanol extracts of Ojayeonjong-hwan (WEO and EEO) and
Jinsimyusaeng-hwan (WEJ and EEJ) showed superior radical scavenging ability
compared to water and 70% ethanol extracts of Gagambang (WEG and EEG).
2. In the results of reducing power using the FRAP method, Ojayeonjong-hwan
and Jinsimyusaeng-hwan showed relatively superior antioxidant activity compared to
Gagambang, which was shown to be dependent on the total phenol content
contained in the extracts.
3. In comparison of the protective effect against H2O2-induced oxidative stress in
C2C12 myoblasts, water extracts had no significant effect, but the 70% ethanol
extracts inhibited H2O2-mediated cytotoxicity in a concentration-dependent manner.
4. The cytotoxic protective effect of 70% ethanol extracts of Ojayeonjong-hwan
(EEO) and Jinsimyusaeng-hwan (EEJ) gainst oxidative stress in C2C12 myoblasts
was correlated with their inhibitory effects on H2O2-induced apoptosis and cell cycle
arrest.
5. In H2O2-treated C2C12 myoblasts, the apoptosis inhibitory effects of EEO and
EEJ were associated with suppression of mitochondrial dysfunction and DNA
damage.
6. The protective effects of EEO and EEJ against H2O2-induced oxidative stress
in C2C12 myoblasts were directly related to the inhibition of ROS generation, and
EEJ showed superior antioxidant activity than EEO.
In conclusion, although Gagambang, Ojayeonjong-hwan and Jinsimyusaeng-hwan
extracts all have protective potential against oxidative stress, compared to
Gagambang, Ojayeonjong-hwan and Jinsimyusaeng-hwan extracts showed relatively
superior antioxidant activity. In addition, the protective effect against H2O2-induced
oxidative stress in C2C12 myoblasts was superior to the ethanol extracts than the
water extracts, and in particular, the anti-oxidative effect of the Ojayeonjong-hwan
extract was the highest.

목차

Ⅰ. 서 론 ··········································································································································1
Ⅱ. 재료 및 방법 ·····························································································································4
1. 각 처방전 추출물의 제조 ···········································································································4
2. DPPH 라디칼 소거 활성 측정 ··································································································6
3. ABTS 라디칼 소거 활성 측정 ·································································································7
4. 총 페놀성 화합물 함량 측정 ·····································································································7
5. FRAP 활성 측정 ··························································································································8
6. 세포 배양 ·······································································································································8
7. 세포 생존율 측정 ·························································································································8
8. 4'', 6-diamidino-2-phenylindole (DAPI) 형광 염색 ····························································9
9. 세포 주기 측정 ·····························································································································9
10. Annexin V-fluorescein-5-isothiocyanate (FITC) 염색을 통한 세포사멸 분석 ······· 10
11. 총 단백질 분리 및 Western blot 분석 ·············································································10
12. MMP (Δψm) 분석 ···················································································································11
13. 세포 내 ROS 수준의 측정 ····································································································11
14. 통계 분석 ···································································································································12
Ⅲ. 결 과 ········································································································································13
1. 가감방, 오자연종환 및 진심유생환 열수 및 에탄올 추출물의 수율 비교 ·············· 13
2. 가감방, 오자연종환 및 진심유생환 열수 및 에탄올 추출물의 DPPH 자유 라디칼소거 활성 비교 ······13
3. 가감방, 오자연종환 및 진심유생환 열수 및 에탄올 추출물의 ABTS 양이온 라디칼소거 활성 비교 ···16
4. 가감방, 오자연종환 및 진심유생환 열수 및 에탄올 추출물의 총 페놀 함량 및 환원력 비교 ·····18
5. C2C12 세포에서 세 종류의 열수 추출물과 H2O2의 세포 독성 평가 ·····························20
6. C2C12 세포에서 H2O2로 유도된 산화적 스트레스에 대한 WEG, WEO 및 WEJ의 영향 평가····23
7. C2C12 세포에서 H2O2로 유도된 산화적 스트레스에 대한 EEG의 영향 ·············· 23
8. C2C12 세포에서 H2O2로 유도된 세포 주기의 변화에 대한 EEG의 영향 ············ 25
9. C2C12 세포에서 annexin V를 이용한 H2O2 유도 세포사멸에 미치는 EEG의 영향 평가 ···28
10. C2C12 세포에서 H2O2에 의한 미토콘드리아 기능 손상에 대한 EEG의 영향 ·········· 31
11. C2C12 세포에서 H2O2로 유도된 ROS 수준 증가에 대한 EEG의 영향 ····················33
12. C2C12 세포에서 H2O2로 유도된 산화적 스트레스에 대한 EEO의 영향 ·················· 35
13. C2C12 세포에서 H2O2로 유도된 세포 주기의 변화에 대한 EEO의 영향 ·················· 37
14. C2C12 세포에서 annexin V를 이용한 H2O2 유도 세포사멸에 미치는 EEO의 영향 평가 ···37
15. C2C12 세포에서 H2O2에 의한 미토콘드리아 기능 손상에 미치는 EEO의 영향 ···· 42
16. C2C12 세포에서 H2O2에 의해 증가된 ROS 수준에 대한 EEO의 영향 ······················42
17. C2C12 세포에서 H2O2로 유도된 산화적 스트레스에 대한 EEJ의 영향 ····················· 45
18. C2C12 세포에서 H2O2로 유도된 세포 주기의 변화에 미치는 EEJ의 영향 ··············· 45
19. C2C12 세포에서 H2O2에 의해 유도되는 세포사멸에 미치는 EEJ의 영향 ················· 49
20. C2C12 세포에서 H2O2에 의한 미토콘드리아 기능 손상에 대한 EEJ의 영향 ··········· 52
21. C2C12 세포에서 H2O2로 유도된 ROS 수준 증가에 대한 EEJ의 영향 ·······················52
Ⅳ. 고 찰 ········································································································································55
Ⅴ. 결 론 ········································································································································65
참고문헌 ···········································································································································67
Abstract (In English) ················································································································78

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