Skin, the largest organ in human body, plays an important role by preventing the loss of body fluids and acting as a barrier against external pathogens. Successful regeneration of skin deep wounds (such as skin cancer, burn wound, and ulcer) is still very challenging due to loss of natural healing functions. To overcome the limitations, skin wound patches using biomaterials or stem cells have developed in the field of regenerative medicine. Among those, Decellularized extracellular matrix (ECM), an organized mixture of natural components (such as collagen, fibronectin, laminin, and proteoglycan), has been a very promising resource for effective tissue regeneration. Hence, cell-derived matrix (CDM), has a highly complex network of macromolecules that can mimic native tissue microenvironments, also has been used in tissue engineering research due to its biophysical and biological properties. In this study, we report the three types of novel CDM-based skin patches for examining a deep wound repair. First of all, we fabricated a dressing type ECM patch that physically combines fibroblast-derived matrix (FDM) and polyvinyl alcohol (PVA) hydrogel. In addition, human mesenchymal stem cells (hMSCs) are transplanted on the dressing type ECM patch to improve therapeutic effect on wound healing. Second, we prepared a paste type ECM patch that comprises a mixture of CDM, thermo-sensitive polymer (Pluronic F127), and natural polymer (hyaluronic acid). Lastly, we developed a scaffold-free type ECM patch that forms only CDM without any polymer scaffold. When the three types of CDM-based skin patches apply into full-thickness mouse skin wound model, the results revealed that the CDM-based skin patches improve the regeneration of epidermis/dermis (in the wound region), neovessel formation, and skin appendages (hair follicle and gland) in comparison with other experimental groups. These results demonstrate that ECM components induce an effective wound recovery by maximizing the efficacy of tissue regeneration. Taken together, our engineered ECM patches have a strong regenerative potential toward advanced wound healing that is nearly close to normal skin tissue level. Furthermore, we expect that our research utilizing CDM has a possibility to expand that can contribute to other tissue regeneration such as bone, cartilage, heart, and cornea.