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논문 기본 정보

자료유형
학위논문
저자정보

Md Hafizur Rahman (강원대학교, 강원대학교 대학원)

지도교수
Young-seok Lim
발행연도
2021
저작권
강원대학교 논문은 저작권에 의해 보호받습니다.

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이 논문의 연구 히스토리 (2)

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Potato (Solanum tuberosum L.) is one of the staple food in many countries but its production is hampered because of the low fertility of the soil different types of diseases, and lack of quality seeds. Due to the lacking of supply of virus-free seed, most of the farmers reuse their seed year after year. Due to seed degeneration and infection by various potato-borne diseases, it may cause severe yield loss. To mitigation, the lack of quality seeds, tissue culture links with soilless culture systems like hydroponics and aeroponics systems should be tried and that would be the supreme deal to enhance the tuber yield.
This study was comprised of two different production systems such as aeroponics and substrate culture under natural and artificial LED light, respectively of three potato varieties Golden king(V 48) and Happy king(N 198) Chungang (V 41) in the greenhouse conditions consequently in three different studies. The first study was conducted in two different locations in semi-controlled greenhouses in an aeroponics system. The first study was conducted to evaluate the optimum planting time (viz. early and late) of potato seed tuber production in an aeroponic system for golden king and happy king. The potato was grown at two different times early and late fall.
The second study was to analyze the effect of foliar application of chemicals on potato tuber yield and photosynthetic and morphological performance. Nine chemical treatments was applied in this study including one control , T1=Salicilic Acid (SA) 300 ppm , T2= Meta Topoline(MT) 50 ppm, T3= Meta Topoline+Indolce 3 Acetic Acid (MT+IAA) 50 ppm + 50 ppm, T4= Chlorocholine chloride (CCC) 2000 ppm, T5= Putrescine (50 ppm), T6=GA3(100 ppm), T7=GA3(200 ppm), T8=GA3(300 ppm), T9= control.
The third study was conducted to analyze the effect of artificial light on potato growth characteristics and tuber yield of two potao varieties Golden king(V 48) and Chungang (V 41). The potatoes plant was treated by six artificial LEDs light with low light intensity 100 μmolm?2, L1 (R:B:Fr), L2 (R:BW), L3 B:Fr), L4 (B:W), L5 (R:Fr), L6 (R:W) and L7 plant in natural light.
The fourth study was conducted to evaluate the effect of artificial light on potato growth characteristics and tuber yield and secondary metabolites, variety Golden king(V 48) in the aeroponic culture system. This study was conducted under in 8 different LEDs lights , L1 (natural light) L2 (R:B), L3(R:G:B), L4(R:B:Fr), L5(R:G:B:Fr), L6(R:G:B:Fr:UV), L7(R:B:Fr:UV), L8(R:B:Fr:W), L9(R:B:Fr:W:UV) light intensity 300 μmolm?2.
In study 1, the growth characteristics and tuber yield of both potato varieties were significantly increased in diverse growing times. But in late fall temperature, 3-14°C quite cold outside potato growth and tuber yield is minimal and two studies were conducted in two different locations. For the vegetative growth stage and tuberization growth stage, 2 different nutrient solution was supplied in the plant grown in location 1, withdrawal nitrogen. Whereas, vegetative growth nutrient was supplied in location 2 in the tuber bulking period. Transplanted early and late fall Golden king and Happy king were harvested 3 periods 50, 70, and 90 days after transplantation. Golden king early transplantation in different two locations, 12±0.94 and 11±0.81, 16±0.81 and 14±0.81, 21.66±2.05 and 17.66±1.24 per plant in location 1 and 2 respectively. And the variety Happy king, 9.33±0.47 and 8.33±1.24, 14.66±0.47 and 10.66±2.05, 17±1.69 and 11.66±1.24 per plant in location 1 and 2 consequently. Golden king late Fall transplantation in different two locations, in 2 locations, 9.33±0.74 and 6±0.81, 11.66±0.47 and 10.66±2.05, 12.41±1.69 and 11.66±1.24 per plant severally. Besides, variety Happy king, 4.33±1.24 and 4±0.81, 4.33±1.24 and 4±0.81, 6.26±1.63 and 5±0.81 per plant progressively.
In treatment T8=GA3(300 ppm) showed the highest tuber number around 24 tuber plant though tuber fresh weight was minimal. However, GA3 treated tuber looks abnormal it was changed from the original variety tuber shape. T4= Chlorocholine chloride (CCC) 2000 ppm, represent a significant tuber yield compare to other treatment and heighst tuber fresh weight.
In study 3, in substrate soil, six LED light, intensity 100 μmolm?2, L1 (R:B:Fr), L2 (R:BW), L3 B:Fr), L4 (B:W), L5 (R:Fr), L6 (R:W) and L7 plant in natural light. Best tuber has been recoded under L2 Red:Blue:White, light 6 tuber/plant variety Chungang(V 41) and 5 tuber/plant variety Golden king(V 48) and no tuber have been found under light L6(Red:White) in two varieties.
In the fourth study, potato variety Golden king has been treated under in 8 different LEDs lights, L1 (natural light) L2 (R:B), L3(R:G:B), L4(R:B:Fr), L5(R:G:B:Fr), L6(R:G:B:Fr:UV), L7(R:B:Fr:UV), L8(R:B:Fr:W), L9(R:B:Fr:W:UV) light intensity 300 μmolm?2, Under lights treatmentL4( R:B:Fr)has been recorded highest number tuber/plant around 22 on the other hand under light treatment L6( R:G:B:Fr: UV) recorded lowest number tuber 6/plant

목차

CONTENTS
Abstract I
List of figures VII
List of tables IX
List of abbreviations X
Chapter 1
General Introduction…………………………………………………………….1
Chapter 2
Exp. 1: Optimization of potato tuber harvesting by analyzing growth, development, and
partitioning in two planting time growing in the aeroponic system
2.1 Introduction 4
2.2 Materials and Methods
2.2.1 Plant Material and Growing Conditions 5
2.2.2 Growing area………………………………………………………………………6
2.2.3 Growing nutrient solutions 7
2.2.4 Transplantation times 7
2.2.5 Growth response 7
2.2.6 Plant photosynthetic pigments 7
2.2.7 Dry matter content(%) 8
2.3 Result
2.3.1 Analysis of plant growth response 10
2.3.2 Analysis of shoot, root and stolon dry matter content……………………………11
2.3.3 plant photosynthetic pigments ……………………………………………….….14
2.3.4 Tuber yield 50 DAT…………………………………………………………...…15
2.3.5 Tuber grading 50 DAT………………………………………………………….16
2.3.6 Tuber yield 70 DAT…….……………………………………………………….17
2.3.7 Tuber grading 70 DAT…………………………………………………………..19
2.3.8 Tuber yield 90 DAT……………………………………………………………..20
2.3.9 Tuber grading 90 DAT…………………………………………………….……22
2.4 Discussions
2.4.1 plant growth response…………………………………………………………………23
2.4.2 plant dry matter content……………………………………………………………….23
2.4.3 Plant photosynthetic pigments………………………………………………………...24
2.4.4 Tuber yield…………………………………………………………………………….24
Chapter 3
Exp: Analysis of the effect of the chemical application on potato tuber yield
3.1 Introduction……………………………………………………………………………………25
3.2 Materials and methods
3.2.1 Plant Material and Growing Conditions ………………………………………………..27
3.2.2 Experimental treatments………………………………………………………………...27
3.2.3 Nutrients solutions…………………………………………………………………….…27
3.2.4 Growth Response………………………………………………………………………..27
3.2.5 Plant photosynthetic Pigments……………………………………………………………27
3.2.5 Determination of net photosynthetic rate, transpiration rate, stomatal conductance, and The photosynthetic water use efficiency……………………………………………………………28
3.2.6 Determination of total soluble carbohydrate and total soluble sucrose content…………28
3.3 Result and discussion
3.3.1 Plant morphological characteristics………………………………………………………29
3.3.2 Plant photosynthetic pigments……………………………………………………………30
3.3.3 Plant photosynthetic and transpiration rate……………………………………………….31
3.3.4 Plant stomatal conductance, and water use efficiency……………………………………32
3.3.5 Plant total soluble carbohydrate and total soluble sugar content………………………….33
3.3.6 Tuber yield………………………………………………………………………………...34
Chapter 4
Exp. 2: Potato mini tuber seed production under low light-emitting Diodes (LEDs) in
substrate soil and green-house conditions
4.1 Introduction…………………………………………………………………………….35
4.2 Materials and Methods
4.2.1 Light-emitting diode (LEDs) settings 37
4.2.2 Plant materials and growth conditions … 38
4.2.3 Determination of plant growth characteristics 38
4.2.4 Photosynthetic pigments 38
4.2.5 Determination of sucrose and total carbohydrate content 38
4.3 Result
4.3.1 Effect of light-emitting diodes (LEDs) on plant growth 41
4.3.2 Effect of light-emitting diodes (LEDs) on plant photosynthetic pigments 43
4.3.3 Effect of light-emitting diodes (LEDs) on plant secondary metabolites ….……..44
4.3.4 Effect of LEDs light on tuber yield………………………………………………44
4.4 Discussion………………………………………………………………………………….45
Chapter 5
Exp. 3: Light Emitting Diodes(LEDs) pre-treatment on potato seed tuber production in an
aeroponic system
5.1 Introduction 47
5.2 Materials and Methods
5.2.1 Light-Emitting Diode (LED) Settings 49
5.2.2 Plant materials and growth conditions … 50
5.2.3 Plant growing nutrients under LEDs light……………………………………….50
5.2.4 Effect of LEDs light on plant growth 50
5.2.5 Effect of LEDs light on plant photosynthetic pigment 50
5.2.6 Effect of LEDs light on plant secondary metabolites content 51
5.2.7 Analysis of tuber yield 51
5.3 Result
5.3.1 Effect of LED lights on potato plant growth in the aeroponics system………….53
5.3.2 Effect of LED lights on Chlorophyll and carotenoids content in potato plants in the aeroponics system 54
5.3.3 Effect of LED lights on secondary metabolites in potato plants and tuber in the aeroponics system ……………..……...……………………………………….…55
5.3.4 Effect of different LEDs Lights on potato tuber yield in the aeroponic system …………………………………………………………………………..………. 56
5.4 Discussion………………………………………………………………………………56
Chapter 6
Conclusion 59
Chapter 7
References…………………………………………………………………………………60
List of Figures
Chapter 1
Fig 1: The photograph of potato seed production in the aeroponic culture system……….1
Fig 2: Potato seed production under LED light……………………………………………2
Chapter 2
Fig 3: 50 DAT shoot, root, stolon dry matter content in different growing times, and locations,
location 1(A,B) and location 2 (C,D)……………………………………………………...10
Fig 4: 50 DAT Tuber Yield in different growing times and locations, location 1(A,B) and location 2 (C,D)…………………………………………………………………………....15
Fig 5: 50 DAT Tuber Grading in different growing times and locations, location 1(A,B) and location 2 (C,D)……………………………………………………………………………16
Fig 6: 70 DAT Tuber Yield in different growing times and locations, location 1(A,B) and location 2 (C,D)……………………………………………………………………………17
Fig 7: 70 Tuber Grading in different growing times and locations, location 1(A,B) and location 2 (C,D)…………………………………………………………………………….19
Fig 8: 90 DAT Tuber Yield in different growing times and locations, location 1(A,B) and location 2 (C,D)…………………………………………………………………………….20
Fig 9: 90 Tuber Grading in different growing times and locations, location 1(A,B) and location 2 (C,D)…………………………………………………………………………….22
Chapter 3
Fig 10. Effect of foliar application of chemicals on morphological characteristics of potato………………………………………………………………………………………29
Fig 11: Effect of chemicals application on photosynthetic pigments……………………..30
Fig 12. Effect of chemicals application on photosynthetic and transpiration rate……..….31
Fig 13. Effect of chemicals application on stomatal conductance and water use efficiency………………………………………………………………………………….32
Fig 14. Effect of chemicals application on total soluble carbohydrate content and total soluble sugar content…………….………………………………………………………..33
Fig 15. Effect of chemicals application on potato tuber yield…………………………....34
Chapter 4
Fig 16: Effect of LEDs light treatment on the Total carbohydrate content and sucrose content of potato plants in greenhouse condition. …………………………………………………43
Fig 17: Effects of LEDs light on the yield of potato tubers...................................................44
Chapter 5
Fig 18: Effect of LED lights on Chlorophyll and carotenoids content in potato plants in the aeroponic system……………………………………………………………….………….54
Fig 19: Effect of LED lights on secondary metabolites in potato plants and tuber in the aeroponic system…………………………………………………………………………..55
Fig 20: Fig 1: Effect of different LEDs Lights on potato tuber yield in the aeroponic system…………………………………………………………………………………..…56
List of Tables
Chapter 2:
Table 1. Bellow table represent the growing nutrient solution formulations for vegetative and tubr bulking periods…………………………………………………………………………...6
Table 2: 50 Day After Transplantation (DAT) Plant Morphological Data in different growing times and locations…………………………………………………………………………….9
Table 3: 50 DAT plant photosynthetic pigments chlorophyll a, chlorophyll b, total chlorophyll, and Carotenoid Content, in different locations and growing times……………..……………13
Chapter 4:
Table 4: Light spectrum combination……………………………………………………….37
Table 5: Effect of Light Emitting Diodes (LEDs) on the growth parameters of potato plants in greenhouse conditions……………….………………………………………………………40
Table 6: Effect of LEDs light treatments on photosynthetic pigments, total chlorophyll, and carotenoids……………………………....……………………………………………..…….42
Chapter 5:
Table 7. Light spectrum for potato production in the aeroponic system……………….……50
Table 8: Effect of LED lights on potato plant growth in the aeroponic system…………………………………………………………………………………………52
Abbreviations
DAT: Day after transplantation
EP: early plantation
LP: late plantation
MT: marketable tuber
MTN: marketable tuber number
TTN: total tuber number
PH: plant height
LN: leaf number
LL: leaf length
RL: root length
ST: stolon length
LW: leaf width
RBF: Red: Blue: Far-red
RBW: Red: Blue: White
RF: Red: far-red
BF: Blue: Far-red
RW: Red: White
BW: Blue: White
MT: Meta topline
SA: Salicylic acid
CCC: Chlorocholine chloride
IAA: Indolce 3 acitic acid
GA: Gibrelic acid
WUE: Water use efficiency
TSC: Total soluble carbohydrate
TSS: Total soluble sugar

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