목차

I. Introduction
II. Literature review
1. Brassica juncea and sinigrin
2. Obesity
1) Definition and therapy issues
2) Mechanism of obesity
III. Materials and methods
1. Chemicals and antibodies
2. Identification of Brassica juncea
1) PCR using universal primer
3. Analysis of nutritional components
1) Proximate composition analysis
2) Free sugar analysis
3) Fatty acid analysis
4) Mineral analysis
5) Vitamin analysis
6) Amino acid analysis
4. Method validation for determination of sinigrin
1) Analysis of sinigrin content
2) Specificity
3) Accuracy and Precision
4) Linearity
5) LOD and LOQ
5. Standardization of extraction process
1) Preparation of the BJJ extract according to extraction solvent, temperature and time
6. Anti-oxidant activity and bioactive compounds
1) DPPH radical scavenging activity
2) ABTS radical scavenging activity
3) Ferric reducing antioxidant power (FRAP) assay
4) Reducing power assay
5) Oxygen radical absorbance capacity (ORAC) assay
6) Total flavonoid and phenol content
7. Anti-obesity activity
1) Cell culture and XTT assay
2) Oil Red O staining
3) Nitro blue tetrazolium (NBT) assay
4) Western blot analysis
8. Statistical analysis
IV. Results and discussion
1. Identification of Brassica juncea
2. Nutritional components of Brassica juncea cultivated in Jeongseon
1) Proximate composition
2) Free sugar composition
3) Fatty acid composition
4) Mineral composition
5) Vitamin composition
6) Amino acid composition
3. Method validation for determination of sinigrin
1) Specificity
2) Accuracy and precision, linearity, LOD and LOQ
4. Changes in the content of sinigrin by extraction solvent, temperature and time
5. Anti-oxidant activity and bioactive compounds of standardized Brassica juncea cultivated in Jeongseon extract
1) DPPH radical scavenging activity
2) ABTS radical scavenging activity
3) Ferric reducing antioxidant power (FRAP)
4) Reducing power
5) Oxygen radical absorbance capacity (ORAC) Total flavonoid and phenol content
6) Total flavonoid and phenol content
6. Anti-obesity activity of standardized Brassica juncea cultivated in Jeongseon extract
1) Effect of BJJ and sinigrin on the cell viability
2) Effect of BJJ and sinigrin on the lipid accumulation and ROS production
3) Effect of BJJ and sinigrin on the protein expression of adipogenic transcription factors
4) Effect of BJJ and sinigrin on the protein expression of lipid synthesis factors
5) Effect of BJJ and sinigrin on the protein expression of lipolytic effect factors
6) Effect of BJJ and sinigrin on the protein expression of energy metabolism factors
V. Conclusion
VI. References
VII. Abstract in Korean