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학위논문
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최은식 (경남과학기술대학교, 경남과학기술대학교 대학원)

지도교수
손시환
발행연도
2021
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경남과학기술대학교 논문은 저작권에 의해 보호받습니다.

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이 논문의 연구 히스토리 (5)

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In the poultry industry, hatching performance and vitality of chicks are important factors affecting productivity. No matter how excellent breeds are developed, poor hatching performance or poor health of the hatching chickens will adversely affect the productivity of chickens. In order to improve hatching performance and vitality of early chick, it is necessary to analyze various factors affecting hatching performance and chicks vitality. Therefore, we analyzed the effects of chicken breed, laying age, and egg storage period on hatching performance and hatched chicks vitality in Korean native chickens. Using Hwanggalsaek Jaeraejong, Korean Rhode Island Red, and Korean White Leghorn chicken breeds, the eggs collected in early laying period (27 to 29 weeks old) and late laying period (50 to 52 weeks old) were stored for 3, 7 and 14 days, respectively, and then hatched and chicks were produced. The fertility, hatchability, embryonic mortality and incubation duration were investigated. Body weight after hatching, body weight at 7d, weight gain, feed intake, survival rate and organ weight in hatched chicks were measured and analyzed. In addition, the gene expression levels of HSP 70, HSP 90α, HSP 90β, and IL-6, relative length of telomere and H/L ratio were also analyzed using blood. The results showed that the chicken breed, laying age, and egg storage period had a significant effect on hatching performance. The longer the egg storage period, the significantly lower hatchability and longer incubation time. Chickens produced at the early laying period had lower fertility rates but higher hatchability than those produced at late laying periods. The incubation time of the Hwanggalsaek Jaeraejong was the shortest, and the Korean Rhode Island Red breed had the lowest hatching performance. In the effects of breed, laying age, and egg storage period on the chick vitality, the survival rate and weight gain of the Hwanggalsaek Jaeraejong chicks were superior, while the Korean Leghorn chicks had the lowest performance. Although the chicks produced at the early laying period had low performance in survival rate and hatching weight, the weight at 7d and weight gain were excellent. In the organ weight to body weight ratio, of Korean Leghorn chicks and the chicks produced at the early laying period were relatively high in liver and low in heart and spleen. The Korean Leghorn chicks and the chicks produced at the late laying period had relatively shorter telomere length, while gene expression levels of HSP 70, HSP 90α, HSP 90β and IL-6 were higher.
In conclusion, there were differences in hatching performance and vitality of hatched chicks according to breeds of Korean native chicken. The laying age in chickens also affected the hatching performance and vitality of chicks. The hatching performance of the late laying period eggs was lower than that of the early laying period eggs, and the vitality of the hatched chicks was also poor. The longer the storage period of eggs, the lower the hatching performance, but it did not significantly affect the vitality of the hatched chicks. Therefore, to obtain good hatching performance and healthy chicks, it is desirable to avoid the use of eggs produced in the old breeding flock, and to shorten the storage period of eggs. In addition, it is necessary to manage breed-specific hatching conditions and to select a chicken breed with strong vitality.

목차

목 차
목 차 ····································································ⅰ
표 목차 ···································································ⅲ
그림목차 ········································································ⅴ
Ⅰ. 서 론 ············································································ 1
Ⅱ. 연구사 ·································································· 3
1. 병아리의 부화 성적에 미치는 요인 ································· 3
2. 병아리의 능력에 미치는 요인 ·············································· 6
3. 병아리의 강건성과 관련된 장기 및 지표 ·············································· 8
Ⅲ. 종계의 산란 연령과 종란의 보관 기간 및 품종에 따른 부화 성적 비교 분석 ································································································· 12
1. 서론 ······················································································· 12
2. 재료 및 방법 ············································································· 14
1) 공시계 및 실험설계 ·································································· 14
2) 부화 작업 ··············································································· 14
3) 조사 항목 ················································································ 15
(1) 수정률 ················································································· 15
(2) 배자발육중지율 ······································································· 16
(3) 부화율 ················································································· 16
4) 통계 분석 ················································································ 16
3. 결과 ······················································································· 17
1) 종계의 품종에 따른 부화 성적 ······················································· 20
2) 종계의 산란 시 연령 ··································································· 24
3) 종란의 보관 기간 ······································································· 27
4. 고찰 ······················································································· 29
Ⅳ. 종계의 산란 연령과 종란의 보관 기간 및 품종에 따른 병아리의 강건성 비교 분석 ·························································································· 32
1. 서론 ························································································· 32
2. 재료 및 방법 ········································································· 34
1) 공시계 및 실험설계 ································································· 34
2) 부화 작업 ·········································································· 34
3) 사양 관리 ·········································································· 34
4) 조사 항목 ·········································································· 35
(1) 병아리의 일반능력 ····························································· 35
(2) 장기 샘플 채취 ································································· 35
5) qRT-PCR을 이용한 telomere, HSP 및 IL-6 분석 ························· 35
(1) RNA 추출 및 cDNA 합성 ··················································· 35
(2) Real-time PCR 수행 ······················································· 36
6) Heterophil to leukocyte ratio 분석 ········································· 36
7) 통계 분석 ·········································································· 37
3. 결과 및 고찰 ······································································· 39
1) 병아리의 생존율 및 발육 특성에 미치는 영향 ······························· 39
2) 병아리의 장기 무게에 미치는 영향 ············································ 45
3) 병아리의 면역 및 생리활성도에 미치는 영향 ································· 49
4) 병아리의 스트레스 반응 정도에 미치는 영향 ································· 52
Ⅴ. 결 론 ·············································································· 55
Ⅵ. 참고문헌 ················································································ 57
Ⅶ. 국문초록 ············································································ 71
Ⅷ. Abstract ··········································································· 73

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