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Contents····································································································i
List of Tables···························································································iv
Lists of Figures·························································································v
Abstract····································································································1
서론··········································································································3
재료 및 방법····························································································10
1. 염분농도 설정을 위한 예비실험···························································10
1.1 실험어·························································································10
1.2 염분농도 설정 ·············································································11
2. 혈액채취···························································································12
2.1 혈액생화학적분석··········································································13
2.2 Hematocrit··················································································13
2.3 혈장 Cortisol activity····································································14
2.4 혈장 superoxide dismutase(SOD) activity·······································16
3. 조직채취····························································································18
3.1 명태 조직 샘플링··········································································18
3.2 명태 Total RNA extraction····························································19
3.3 RNA 농도 측정············································································20
3.4 cDNA합성····················································································21
3.5 프라이머 디자인 및 PCR·······························································21
3.6 Gel purification············································································24
3.7 Ligation 및 Transformation, Miniprep············································25
3.8 염기서열분석················································································26
3.9 유전자 정량 분석(Real-time PCR)··················································27
4. 통계분석···························································································28
결과········································································································29
1. 염분변화에 따른 생존률 및 염분결정····················································29
2. 혈액 생리학적 분석············································································30
2.1 혈장 SOD 활성··········································································30
2.2 Ht 와 Hb 농도··········································································32
2.3 GOP, GPT 및 TP·······································································34
2.4 혈장 Cortisol 및 Glucose···························································36
3. 유전자 발현······················································································38
3.1 염분변화에 따른 Steroidogenic acute regulatory protein (StAR) mRNA
의 변화······················································································38
3.2 염분변화에 따른 Glucocorticoid receptor (GR) mRNA의 변화·······40
3.3 염분변화에 따른 Heat shock protein 70 (Hsp70) mRNA의 변화·····42
고찰········································································································44
요약········································································································52
참고문헌··································································································54