목차

CHAPTER I 1
General introduction and literature review 1
1.1. General introduction 2
1.2. Literature review 6
1.3. References 9
CHAPTER Ⅱ 14
Characterization of fruit quality attributes of four Korean apple cultivars at harvest and their ethylene patterns during shelf-life storage condition 14
2.1. Abstract 15
2.2. Introduction 16
2.3. Materials and methods 17
2.3.1. Plant materials 17
2.3.2. Assessment of fruit quality attributes 17
2.4. Results 18
2.4.1. Fruit quality attributes at harvest 18
2.4.2. Ethylene production stored at 20C shelf-life condition 19
2.5. Discussion 21
2.6. References 22
CHAPTER Ⅲ 25
Responses of fruit quality attributes of four Korean apple cultivars to 1-methylcyclopropene (1-MCP) treatment during cold storage 25
3.1. Abstract 26
3.2. Introduction 27
3.3. Materials and methods 28
3.3.1. Plant materials and treatment 28
3.3.2. Assessment of fruit quality attributes 28
3.3.3. Statistical analysis 29
3.4. Results 29
3.4.1. Fruit quality attributes during cold storage 29
3.4.2. Pearson''s correlation coefficient analysis 41
3.5. Discussion 43
3.6. References 46
CHAPTER Ⅳ 50
Fruit softening and cell wall metabolism of four Korean apple cultivars to 1-methylcyclopropene (1-MCP) treatment during cold storage 50
4.1. Abstract 51
4.2. Introduction 52
4.3. Materials and methods 54
4.3.1. Plant materials and treatments 54
4.3.2. Extraction and measurement of cell wall components 55
4.3.3. Extraction and measurement of activities of cell wall-degrading enzymes 56
4.3.4. Extraction and measurement of uronic acid and total sugar contents 57
4.3.5. Extraction and measurement of molecular weight of polyuronides and total sugar content by gel permeation chromatography 58
4.3.6. Extraction and measurement of non-cellulosic neutral sugar compositions 58
4.3.7. Statistical analysis 59
4.4. Results 60
4.4.1. Cell wall components 60
4.4.2. Cell wall-degrading enzymes 64
4.4.3. Uronic acid and total sugar contents 70
4.4.4. Gel permeation chromatography 72
4.4.5. Non-cellulosic neutral sugar compositions 82
4.4.6. Pearson''s correlation coefficient analysis 88
4.5. Discussion 94
4.6. References 99
CHAPTER Ⅴ 105
Expression analysis of softening-related genes of four Korean apple cultivars to 1-methylcyclopropene (1-MCP) treatment during cold storage 105
5.1. Abstract 106
5.2. Introduction 107
5.3. Materials and methods 108
5.3.1. Plant materials and treatments 108
5.3.2. Transcriptional analysis of genes encoding cell wall- degrading enzymes 109
5.3.3. Statistical analysis 109
5.4. Results 111
5.4.1. Expression patterns of the genes associated with cell wall degradation 111
5.4.2. Pearson''s correlation coefficient analysis 117
5.5. Discussion 122
5.6. References 124
Summary 127
Abstract (English) 130
Abstract (Korean) 132