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논문 기본 정보

자료유형
학위논문
저자정보

김준호, Kim, Jun Ho (충북대학교, 충북대학교 대학원)

지도교수
이범준
발행연도
2019
저작권
충북대학교 논문은 저작권에 의해 보호받습니다.

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이 논문의 연구 히스토리 (2)

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Clostridium perfringens (C. perfringens) may cause diarrhea and enterotoxemia in adult and young livestock, leading to problems in the production and management of farms. Four hundred fecal samples were collected from 25 goat farms located in Gyeongsangbuk-do Province in the Republic of Korea. Sixteen C. perfringens strains were isolates from fecal samples, and the isolates were identified as type A (n=11) and type D (n=5). Additionally, α- and ε-toxin genes were detected in 16 and 5 strains by PCR, respectively, and the enterotoxin gene was presented in 2 strains. The antibiotic susceptibility test was performed using the disk diffusion method and E-test method. In the disk diffusion method, ampicillin (n=16) and chloramphenicol (n=15) were highly susceptible to 16 C. perfringens isolates. In the E-test method, ampicillin, amoxicillin, amoxicillin/clavulanic acid and meropenem were susceptible to more than 14 of 16 C. perfringens isolates. This study indicates that administration of antibiotics such as ampicillin, amoxicillin/clavulanic acid and meropenem can prevent and treat C.perfringens infections in goats.

목차

Ⅰ.INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 4
1.Sampling 4
2. Isolation and identification of C. perfringens 4
3. C. perfringens DNA extraction 5
4. PCR amplification for C. perfringens toxin, type 6
5. Target strain in antibiotic susceptibility test 10
6. Disk diffusion method 10
7. M.I.C.Evaluator strips test(E-test) method 11
Ⅲ. RESULTS 15
1. Isolation and identification of C. perfringens 15
2. Toxins and types of C. perfringens 15
3. Antibiotic susceptibility by disk diffusion method 16
4. Antibiotic susceptibility by E-test method 16
Ⅳ. DISCUSSION 22
ACKNOWLEDGEMENTS 25
REFERENCES 26
SUMMARY IN KOREAN 33

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