The purpose of this study was to investigate the effect of Phellinus linteus Cheonghyeol plus (PLCP) on Inhibition of Antioxidant and Inflammatory Factor Expression Associated with Dyslipidemia in Human umbilical vein endothelial cells (HUVECs). The scavenging activity of DPPH radical and ABTS radical of PLCP was measured in HUVEC. The expression levels of NF-κB, p-IκBα, ERK, JNK, and p38 proteins were measured after treating with TNF-α in HUVEC. The expression levels of MCP-1, ICAM-1, and VCAM-1 mRNA were measured using qRT-PCR after treatment with TNF-α in HUVEC, and expression levels of MCP-1, ICAM-1, and VCAM-1 biomarkers using ELISA assay after treatment with TNF-α in HUVEC, were measured.

The results were as follows:

1. PLCP increases DPPH and ABTS radical scavenging activity in a concentration dependent manner.

2. PLCP significantly decreased the concentration of NF-κB, p-IκBα, ERK, JNK protein compared to the control at concentrations of 100 ㎍/㎖ or more, and significantly decreased concentration of p38 protein at all concentrations.

3. PLCP significantly decreased MCP-1 mRNA expression levels at 100㎍/㎖ or more compared to the control. ICAM-1 and VCAM-1 mRNA expression levels were significantly reduced at all concentrations compared to the control. MCP-1, ICAM-1, protein expression levels were significantly reduced compared to the control at concentrations of 100 ㎍/㎖ or more, and VCAM-1 protein expression levels were significantly reduced at all concentrations.

These results suggest that PLCP has an antioxidant effect, and it has been experimentally confirmed that it can prevent or inhibit inflammatory diseases caused by dyslipidemia due to its anti-inflammatory effect and inflammatory cell migration inhibitory effect in HUVEC.