Objective:
This study was aimed to evaluate anti-inflammatory and antitussive expectoration effectsof Friltillariae Thunbergii Bulbus (FTB) in mouse model.

Materials and Methods:
To evaluate the anti-inflammatory effect of the FTB, we conducted in vitro experiments using RAW264.7 cells. For experimental method, MTT assay and Enzyme-Linked Immunosorbent Assay (ELISA) was were carried out to examine the anti-inflammatory effects of FTB. In addition, the expectorant effect of phenol red secretion, antitussive effect on cough induced by ammonia solution, and leukocyte increase inhibition effect in acute airway inflammation in animal model were confirmed.

Results:
Cytotoxicity and Anti-inflammatory Activity
1. FTB did not show cytotoxicity in the experimental group at 10, 30, 50, 100, 300, 500 ㎍/㎖.
2. FTB significantly inhibited the increase of NO in the experimental groups at 30, 50, 100, 300 and 500 ㎍/㎖ concentrations.
3. FTB significantly inhibited the increase of TNF-α in the experimental groups of 30, 50, 100, 300, and 500 ㎍/㎖.
4. FTB significantly inhibited the increase of IL-6 in the experimental group of 500 ㎍/㎖.

Sputum, Cough and Acute Airway Inflammation Animal Models
1. FTB significantly increased phenol red secretion in the 400 ㎎/㎏ administration group.
2. FTB significantly reduced the number of coughs and significantly increased cough delay time in both 200 and 400 ㎎/㎏ dose groups.
4. FTB decreased the white blood cell count in BALF in the 400 ㎎/㎏ administration group.

Conclusion:
Our study revealed that FTB elicits antitussive and expectorant effect via inhibiting inflammatory cytokine, increasing sputum secretion, suppressing cough and reducing inflammatory cell. We concluded FTB is a highly promising agent for respiratory tract infection with therapeutic opportunities.