The purpose of this study is to examine how a pine needle powder-added diet and swimming exercise influence fat metabolism and inflammatory cytokine in a high cholesterol diet. 42 male Sprague-Dawley rats were made to practice a pine needle powder-added diet and swimming exercise along with a high cholesterol diet for six weeks. The HCE and the HCPEN showed significant decrease in their weight in the sixth week, and according to the results of dispersion analysis, it was found to be significant in the group × week. About the change of intake in the diet, the HC and HCE were found to be significant high, and according to the results of dispersion analysis, it was not significant in the group × week and regarding diet efficiency, the EX, HCPN, and HCPNE were found to be significant high. About the change of fat, abdominal fat was significantly high in the HC and HCPN, and testicle fat was significantly high in the HC, and mesenteric fat did not indicate significant difference. Concerning the results of blood lipids, the TG content was significantly low in the HCPNE, and LDL-C was significantly high in the EX compared with the CON and EX and HDL-C was statistically significantly high in the EX According to the results of fat metabolism, regarding the change of L-FABP expression in liver tissues, the CON and EX indicated a significantly higher expression compared to each of the other groups, and CPT-1 expression did not show significant difference. According to the results of TNF-α concentration in the change of inflammatory cytokine, the HC, HCE, and HCPN indicated significantly higher concentration than the CON and EX, and according to the results of NFκB concentration, no significant difference was found. According to the results of IL-6 concentration, compared with the CON, the EX and HCPNE were found to be significantly low. Therefore, according to the results of this study, reduction in weight, body fat, and blood lipids led by the exercise and pine needle powder-added diet activated AMPK and the acceleration of PPAR-γ, FABP, and CPT-1 as the result of the exercise, and this led to the regulation of fat metabolism. Moreover, regarding the results of pine needle powder intake in the diet, pharmacological compounds contained in pine needles such as phenols and flavonoids are thought to influence fat metabolism and infectious cytokine positively. This author deems that the results of this study provide foundational material regarding the influence of exercise and pine needle powder-added diet’s complex treatment on anti-obesity and anti-inflammatory improvements. Based on the results of this study, this researcher concludes that future physiological research will be able to find pine needles have positive effects as functional food regarding the effects of pine needles and exercise’s complex treatment on energy consumption and metabolic disease.