This study was performed to evaluate the activities of anti-oxidant and anti-inflammatory of water, 70% ethanol extracts of the Ambrosia trifida L. (AT) collected in June and August. The anti-oxidant effects that was measured by electron donating ability, ABTS cation radical scavenging ability assay and Superoxide dismutase (SOD)-like activity. The water and 70% ethanol extracts of AT in June and August showed increased electron donating ability as the concentration increased. Among them, 70% ethanol extract in August showed 82.34% effect at 1,000 ㎍/㎖ and showed the highest antioxidative effect. The ABTS scavenging ability of water and 70% ethanol extracts of AT collected in June and August showed that the scavenging ability of 70% ethanol extract of AT in August at 100 ㎍/㎖ concentration was superior. As a result of SOD-like activity measurement, the monthly AT water extracts showed little activity. On the other hand, the monthly AT 70% ethanol extracts increased the SOD-like activity in a concentration dependent manner. The structure of AT extract was analyzed by the separation of components and NMR. The color development by 10% H2SO4 and FeCl3 solution on the TLC plate and the results of 1H and 13C NMR measurements were compared with the existing literature. Compound 1 was finally identified as styrene-butadiene block copolymer (20 ㎎), compound 2 as erythrodiol (2 ㎎), compound 3 as ursolic acid (8 ㎎) and compound 4 as 3-O-caffeoylquinic acid. The anti- inflammatory activities of AT 70% ethanol extract in August (AT8E) were investigated using Raw 264.7 cells induced by lipopolysaccharide (LPS). Cell toxicity effect of AT8E and compound 1-4 on macrophage cells (RAW 264.7) performed 3-[4,5?dimethyl?thiazol?2?yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay. As a result of cell viability effect measured AT8E and compound 1-4, Each of these was shown 111.5%, 91.68%, 99.24%, 99.43%, 98.12% more with cell viability at 100㎍/㎖ concentration. In order to show anti-inflammatory active, we examined the inhibitory effects on the production of lipopolysaccharide (LPS)-induced NO in RAW 264.7 cells by Griess assay. The result showed that AT8E and compound 1-4 concentration dependent inhibited NO production. The protein expression inhibitory effect of AT8E and compound 1-4 were measured by western blot at 25, 50, 100㎍/㎖ concentrations and the β-actin was used as a positive control. It was confirmed that the amount of protein expression of iNOS and COX-2 increased by LPS in a concentration-dependent manner. As a result of experiment on the effect on MAPKs activity, In RAW 264.7 cells stimulated with LPS, phosphorylation of ERK1 / 2, p38, and c-Jun NH2-terminal kinase (JNK) was inhibited in a concentration-dependent manner in the group treated with AT8E and compound 1-4. AT was extracted using fermentation and anti-inflammatory and whitening activity was verified that its usability as a cosmetic material. The electron donating ability of fermented complex extracts from AT was shown 68.4% at 1,000 ㎍/㎖ concentration. The ABTS+ radical scavenging ability of shown 58.7% at 1,000 ㎍/㎖ concentration. The tyrosinase inhibitory effect which is related to skin-whitening, was 32.35% at the concentration of 1,000 ㎍/㎖. A cell viability test was measured on macrophage cells and melanoma cells by fermented complex extract from AT. The results of the test was showed 82%, 85.2% with cell viability at 100 ㎍/㎖ concentration. In order to show anti-inflammatory active, we examined the inhibitory effects on the production of lipopolysaccharide (LPS)-induced NO in RAW 264.7 cells by Griess assay. The result showed that fermented complex extract from AT by dependence on the concentration inhibited NO production. The protein expression inhibitory effect of fermented complex extract from AT were measured by western blot at 25, 50, 100 ㎍/㎖ concentrations and the β-actin used as a positive control. Consequently, the iNOS and COX-2 protein expression inhibitory effect of fermented complex extract from AT was measured by western blot. And the result showed reduction in the effect by 62.1%, 39.3% at 100 ㎍/㎖ concentration. The phosphorylation of ERK1/2, p38 and JNK protein expression inhibitory effect was decreased by dependence on the concentration. In order to identify whitening effect, According to the results of western blot of fermented complex extract from AT the expression of the MITF, tyrosinase, TRP-1 and TRP-2 protein showed a decrease by 51.14%, 83.77%, 55.4%, 38.6% at 100 ㎍/㎖ concentration. The peak value of 3-O-caffeoylquinic acid, which showed an excellent anti-inflammatory effect on the indicator material, was confirmed by comparing the chromatograms of AT extract and fermented complex extract. As a result of this study, the anti-inflammatory effect of AT extract and standard compounds was verified. The efficacy of anti-inflammatory and whitening effect was verified that the usability of fermented complex extract from AT as a cosmetic material.